Journal of Experimental Botany, Vol. 51, No. 344, pp. 529-538,
March 2000
© 2000 Oxford University Press
Endo-ß-mannanase activity increases in the skin and outer pericarp of tomato fruits during ripening
1 Department of Botany, University of Guelph, Guelph, Ontario N1G 2W1, Canada
2 Department of Plant Physiology, Wageningen Agricultural University, Arboretumlaan 4, 6703 BD Wageningen, The Netherlands
Activity of endo-ß-mannanase increases during ripening of tomato (Lycopersicon esculentum Mill.) fruit of the cultivar Trust. ß-Mannoside mannohydrolase is also present during ripening, but its pattern of activity is different from that of endo-ß-mannanase. The increase in endo-ß-mannanase activity is greatest in the skin, and less in the outer and inner pericarp regions. This enzyme is probably bound to the walls of the outermost cell layers of the fruit during ripening, and it requires a high-salt buffer for effective extraction. The enzyme protein, as detected immunologically on Western blots, is present during the early stages of ripening, before any enzyme activity is detectable. The mRNA for the enzyme is also present at these stages; endo-ß-mannanase may be produced and sequestered in a mature-sized inactive form during early ripening. Most non-ripening mutants of tomato exhibit reduced softening and lower endo-ß-mannanase activity, but a cause-and-effect relationship between the enzyme and ripening is unlikely because some cultivars which ripen normally do not exhibit any endo-ß-mannanase activity in the fruit.
Key words: Tomato, Lycopersicon esculentum, endo-ß-mannanase, softening, ripening.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  R. Schroder, R. G. Atkinson, and R. J. Redgwell Re-interpreting the role of endo-{beta}-mannanases as mannan endotransglycosylase/hydrolases in the plant cell wall Ann. Bot., May 19, 2009; (2009) mcp120v1. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. GONG, G. W. BASSEL, A. WANG, J. S. GREENWOOD, and J. D. BEWLEY The Emergence of Embryos from Hard Seeds is Related to the Structure of the Cell Walls of the Micropylar Endosperm, and not to Endo-{beta}-mannanase Activity Ann. Bot., December 1, 2005; 96(7): 1165 - 1173. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Bargel and C. Neinhuis Tomato (Lycopersicon esculentum Mill.) fruit growth and ripening as related to the biomechanical properties of fruit skin and isolated cuticle J. Exp. Bot., March 1, 2005; 56(413): 1049 - 1060. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Brummell, V. Dal Cin, C. H. Crisosto, and J. M. Labavitch Cell wall metabolism during maturation, ripening and senescence of peach fruit J. Exp. Bot., September 1, 2004; 55(405): 2029 - 2039. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. A. Filichkin, J. M. Leonard, A. Monteros, P.-P. Liu, and H. Nonogaki A Novel Endo-{beta}-Mannanase Gene in Tomato LeMAN5 Is Associated with Anther and Pollen Development Plant Physiology, March 1, 2004; 134(3): 1080 - 1087. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Itai, K. Ishihara, and J. D. Bewley Characterization of expression, and cloning, of {beta}-D-xylosidase and {alpha}-L-arabinofuranosidase in developing and ripening tomato (Lycopersicon esculentum Mill.) fruit J. Exp. Bot., December 1, 2003; 54(393): 2615 - 2622. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Mo and J. D. Bewley The relationship between {beta}-mannosidase and endo-{beta}-mannanase activities in tomato seeds during and following germination: a comparison of seed populations and individual seeds J. Exp. Bot., November 1, 2003; 54(392): 2503 - 2510. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Bourgault and J. D. Bewley Variation in Its C-Terminal Amino Acids Determines Whether Endo-beta -Mannanase Is Active or Inactive in Ripening Tomato Fruits of Different Cultivars Plant Physiology, November 1, 2002; 130(3): 1254 - 1262. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Feurtado, M. Banik, and J. D. Bewley The cloning and characterization of {{alpha}}-galactosidase present during and following germination of tomato (Lycopersicon esculentum Mill.) seed J. Exp. Bot., June 1, 2001; 52(359): 1239 - 1249. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Banik, R. Bourgault, and J. D. Bewley Endo-{beta}-mannanase is present in an inactive form in ripening tomato fruits of the cultivar Walter J. Exp. Bot., January 1, 2001; 52(354): 105 - 111. [Abstract] [Full Text] [PDF]
|
|