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Journal of Experimental Botany, Vol. 51, No. 345, pp. 703-711, April 2000
© 2000 Oxford University Press

Phytochrome regulation of phytochrome A mRNA levels in the model short-day-plant Pharbitis nil

C.E. Carter2, A. Szmidt-Jaworska3, M. Hughes4, B. Thomas1 and S. Jackson1,5

1 Plant Genetics and Biotechnology Department, Horticulture Research International, Wellesbourne, Warwick CV35 9EF, UK
2 Department of Botany, School of Plant Sciences, University of Reading, Whiteknights, Reading RG6 2AS, UK
3 Nicholas Copernicus University, Institute of Biology and nvironmental Protection, Depatment of Plant Physiology and Morphogenesis, Torun, Poland
4 Department of Biochemistry, McMaster University, Hamilton, Ontario, Canada

The exposure of dark-grown Pharbitis nil seedlings to continuous R induces a rapid decrease in PHYA mRNA abundance with a half-life of about 2 h. A 5 min R pulse also induces this decline, and the effect is partially reversible by subsequent FR irradiation, confirming that the regulation of expression is mediated via the Pfr form of a phytochrome. When de-etiolated seedlings are returned to darkness after a W photoperiod, PHYA mRNA slowly reaccumulates from 20% to 50% of the dark level within 24 h. The rate of reaccumulation is greatly accelerated by the removal of Pfr with a FR pulse, resulting in reaccumulation to 100% within approximately 11 h. Without FR irradiation PHYA mRNA expression remains fully repressed for at least 11 h after the end of the photoperiod, suggesting that the controlling Pfr is highly stable.

Key words: Phytochrome A, Pharbitis nil, photoperiodism, short-day plant, far-red light.


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