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Journal of Experimental Botany, Vol. 51, No. 346, pp. 853-863, May 2000
© 2000 Oxford University Press

Controlled induction of GUS marked clonal sectors in Arabidopsis

Nigel J. Kilby1,2,4, Mark J. Fyvie1, R. Allen Sessions3, Gareth J. Davies1,5 and James A.H. Murray1

1 Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QT, UK
2 Laboratory of Plant Physiology and Molecular biology, University of Turku, 20520, Turku, Finland
3 Department of Biology, University of California at San Diego, La Jolla, Ca., 92093–0116 USA

Stably transformed Arabidopsis lines in which GUS marked cell clones are readily produced in response to heat-shock have been established and characterized. Control of GUS activation is achieved by heat-shock-induced FLP recombinase activity which ‘switches on’ expression of a GUS marker gene previously held transcriptionally silent. To obtain efficient GUS sectoring, single insert Arabidopsis lines carrying FLP recombinase under the control of a heat-shock-inducible promoter and an FLP-activatable GUS construct were generated. Analysis of GUS sectoring in lines hemizygous and homozygous for both inserts was conducted after various regimes of heat-shock were given at various developmental stages. It is shown that GUS sectoring events can be efficiently induced in most vegetative, aerial and sexual structures in Arabidopsis. Furthermore, the frequency of sectoring events, sector size and, to some extent, the tissues in which sectors are generated can be readily controlled by choice of the conditions and timing of heat-shock used.

Key words: FLP recombinase, site-specific recombination, Arabidopsis, clonal sectors, lineage analysis.


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