Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Waterworth, W. M.
Right arrow Articles by Bray, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waterworth, W. M.
Right arrow Articles by Bray, C. M.
Agricola
Right arrow Articles by Waterworth, W. M.
Right arrow Articles by Bray, C. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 51, No. 348, pp. 1201-1209, July 2000
© 2000 Oxford University Press

Original papers

The barley scutellar peptide transporter: biochemical characterization and localization to the plasma membrane

Wanda M. Waterworth1, Christopher E. West and Clifford M. Bray

School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK

Thiol-affinity labelling was used to identify and characterize components of the peptide transport system in the barley (Hordeum vulgare) scutellar epithelium. SDS-PAGE and 2D-PAGE in conjunction with fluorography were used to study derivatized proteins. Membrane proteins of 42 kDa and 66 kDa were identified using a strategy devized to label substrate protectable protein with the thiol specific reagent [14C]N-ethylmaleimide (NEM). The scutellar plasma membrane is the anticipated site of transporters involved in the mobilization of endosperm storage reserves in the germinating barley grain. The subcellular localization of these proteins to the plasma membrane was demonstrated by thiol-affinity labelling of high purity plasma membrane vesicles isolated from barley scutellar tissue. A peptide transporter, HvPTR1, specific to the barley scutellum has recently been cloned in this laboratory. A 66 kDa protein, comparable to the predicted molecular mass of HvPTR1, was identified by [14C]NEM labelling studies of Xenopus laevis oocytes expressing HvPTR1 cRNA, but not water injected controls. Peptide antiserum raised to HvPTR1 also cross-reacted with a 66 kDa membrane protein in barley scutellar tissue. This confirms that the 66 kDa protein identified here by thiol-affinity labelling studies is the barley scutellum peptide transporter HvPTR1, and demonstrates that this protein is localized to the plasma membrane of scutellar epithelial cells during germination.

Key words: Peptide transport, scutellum, germination, Hordeum vulgare.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
N. Y. Komarova, K. Thor, A. Gubler, S. Meier, D. Dietrich, A. Weichert, M. Suter Grotemeyer, M. Tegeder, and D. Rentsch
AtPTR1 and AtPTR5 Transport Dipeptides in Planta
Plant Physiology, October 1, 2008; 148(2): 856 - 869.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
N. Sreenivasulu, B. Usadel, A. Winter, V. Radchuk, U. Scholz, N. Stein, W. Weschke, M. Strickert, T. J. Close, M. Stitt, et al.
Barley Grain Maturation and Germination: Metabolic Pathway and Regulatory Network Commonalities and Differences Highlighted by New MapMan/PageMan Profiling Tools
Plant Physiology, April 1, 2008; 146(4): 1738 - 1758.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
W. M. WATERWORTH and C. M. BRAY
Enigma Variations for Peptides and Their Transporters in Higher Plants
Ann. Bot., July 1, 2006; 98(1): 1 - 8.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. M. Waterworth, M. K. Ashley, C. E. West, P. A. Sunderland, and C. M. Bray
A role for phosphorylation in the regulation of the barley scutellar peptide transporter HvPTR1 by amino acids
J. Exp. Bot., June 1, 2005; 56(416): 1545 - 1552.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
M. G. Stacey, S. Koh, J. Becker, and G. Stacey
AtOPT3, a Member of the Oligopeptide Transporter Family, Is Essential for Embryo Development in Arabidopsis
PLANT CELL, November 1, 2002; 14(11): 2799 - 2811.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Koh, A. M. Wiles, J. S. Sharp, F. R. Naider, J. M. Becker, and G. Stacey
An Oligopeptide Transporter Gene Family in Arabidopsis
Plant Physiology, January 1, 2002; 128(1): 21 - 29.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.