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Journal of Experimental Botany, Vol. 51, No. 348, pp. 1229-1236, July 2000
© 2000 Oxford University Press

Original papers

Analysis of bruchid resistance in the wild common bean accession G02771: no evidence for insecticidal activity of arcelin 5

Alain Goossens1,3, Constanza Quintero2, Willy Dillen1,4, Riet De Rycke1, Jose Flower Valor2, Janniek De Clercq1, Marc Van Montagu1,5, Cesar Cardona2 and Geert Angenon1

1 Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie, Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
2 Bean Entomology, Centro Internacional de Agricultura Tropical, Apartado Aéreo 6713, Cali, Colombia

Arcelins are abundant seed storage proteins thought to be implicated in the resistance of wild Phaseolus vulgaris (L.) genotypes against Zabrotes subfasciatus (Boheman), an important storage insect pest of common bean. Here, the insecticidal activity of the arcelin-5 variant that is present in the highly resistant P. vulgaris accession G02771 was investigated. No correlation could be established between the presence of arcelin 5 and the insecticidal effects observed in G02771 seeds. Insect feeding assays with artificial seeds into which purified arcelin-5 protein was incorporated and with transgenic P. acutifolius (A. Gray) seeds in which the arcelin-5 genes were expressed, showed that the presence of arcelin-5 proteins, even at elevated levels, was not sufficient to achieve adequate resistance against Z. subfasciatus. The same might apply to other arcelin variants. Nevertheless, as resistance is clearly closely linked to the presence of the arcelin-1 or arcelin-5 locus, arcelins remain useful markers in breeding programmes aimed at introgressing high levels of resistance to Z. subfasciatus in P. vulgaris cultivars.

Key words: Arcelin, bruchid resistance, Mexican bean weevil, Phaseolus, Zabrotes subfasciatus.


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[Abstract] [Full Text] [PDF]


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