Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (13)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Van der Toorn, A.
Right arrow Articles by Kamenetsky, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van der Toorn, A.
Right arrow Articles by Kamenetsky, R.
Agricola
Right arrow Articles by Van der Toorn, A.
Right arrow Articles by Kamenetsky, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 51, No. 348, pp. 1277-1287, July 2000
© 2000 Oxford University Press

Original papers

Developmental changes and water status in tulip bulbs during storage: visualization by NMR imaging

Annette Van der Toorn1, Hanita Zemah2, Henk Van As1, P. Bendel3 and R. Kamenetsky2,4

1 Department of Biomolecular Sciences, Wageningen University, Wageningen NMR Centre, Wageningen, The Netherlands
2 Department of Ornamental Horticulture, ARO, The Volcani Centre, Bet Dagan, Israel
3 Department of Chemical Services, MR Centre, Weizmann Institute of Science, Rehovot, Israel

Magnetic Resonance Imaging (MRI) and light and scanning electron microscopy (SEM) were used to follow time-dependent morphological changes and changes in water status of tulip bulbs (Tulipa gesneriana L., cv. ‘Apeldoorn’) during bulb storage for 12 weeks at 20 °C (non-chilled) or 4 °C (chilled) and after planting. MR images reflecting the water content, the relaxation times T1 and T2 (or their reciprocal values, the relaxation rates R1 and R2), and the apparent self-diffusion coefficient of water molecules (ADC), were obtained for intact bulbs. After planting, scape elongation and flowering occurred only in chilled bulbs, while elongation in non-chilled bulbs was retarded. Microscopic observations showed different structural components and high heterogeneity of the bulb tissues. MRI revealed the elongation of the flower bud during storage, which was significantly faster in the chilled bulbs. In addition, MRI demonstrated a redistribution of water between different bulb organs, as well as significant differences in the pattern of this redistribution between the chilled and non-chilled bulbs. Generally, R2 relaxation rates became faster in all bulb organs during storage. At the same time, ADC values remained constant in the chilled bulbs, while exhibiting a significant increase in the non-chilled bulbs.

Key words: Tulip bulb, floral development, storage, temperature treatment, geophytes, MRI, SEM.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Tree PhysiolHome page
L. Kalcsits, E. Kendall, S. Silim, and K. Tanino
Magnetic resonance microimaging indicates water diffusion correlates with dormancy induction in cultured hybrid poplar (Populus spp.) buds
Tree Physiol, October 1, 2009; 29(10): 1269 - 1277.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. Van As
Intact plant MRI for the study of cell water relations, membrane permeability, cell-to-cell and long distance water transport
J. Exp. Bot., March 1, 2007; 58(4): 743 - 756.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Bots, F. Vergeldt, M. Wolters-Arts, K. Weterings, H. van As, and C. Mariani
Aquaporins of the PIP2 Class Are Required for Efficient Anther Dehiscence in Tobacco
Plant Physiology, March 1, 2005; 137(3): 1049 - 1056.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. G. van Kilsdonk, K. Nicolay, J. M. Franssen, and C. Kolloffel
Bud abortion in tulip bulbs studied by magnetic resonance imaging
J. Exp. Bot., July 1, 2002; 53(374): 1603 - 1611.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. van der Weerd, M. M.A.E. Claessens, T. Ruttink, F. J. Vergeldt, T. J. Schaafsma, and H. Van As
Quantitative NMR microscopy of osmotic stress responses in maize and pearl millet
J. Exp. Bot., December 1, 2001; 52(365): 2333 - 2343.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.