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Journal of Experimental Botany, Vol. 51, No. 351, pp. 1663-1670, October 2000
© 2000 Oxford University Press

Original Papers

Transport of amino acids (L-valine, L-lysine, L-glutamic acid) and sucrose into plasma membrane vesicles isolated from cotyledons of developing pea seeds

A. de Jong1 and A.C. Borstlap2

Transport Physiology Research Group, Department of Plant Ecology and Evolutionary Biology, Utrecht University, Sorbonnelaan 16, NL-3584 CA Utrecht, The Netherlands

Transport of the amino acids L-valine, L-lysine, and L-glutamic acid and of sucrose was studied in plasma membrane vesicles isolated from developing cotyledons of pea (Pisum sativum L. cv. Marzia). The vesicles were obtained by aqueous polymer two-phase partitioning of a microsomal fraction and the uptake was determined after the imposition of a H+-gradient ({Delta}pH, inside alkaline) and/or an electrical gradient ({Delta}{psi}, inside negative) across the vesicle membrane. In the absence of gradients, a distinct, time-dependent uptake of L-valine was measured, which could be enhanced about 2-fold by the imposition of {Delta}pH. The imposition of {Delta}{psi} stimulated the influx of valine by 20%, both in the absence and in the presence of {Delta}pH. Uptake of L-lysine was more strongly stimulated by {Delta}{psi} than by {Delta}pH, and its {Delta}pH-dependent uptake was enhanced about 6-fold by the simultaneous imposition of {Delta}{psi}. In the absence of gradients the uptake of L-glutamic acid was about 2-fold higher than that of L-valine, but it was not detectably affected by {Delta}pH or {Delta}{psi}. Although the transport of sucrose was very low, a stimulating effect of {Delta}pH could be clearly demonstrated. The results lend further support to the contention that during seed development cotyledonary cells employ H+-symporters for the active uptake of sucrose and amino acids.

Key words: Amino acids, cotyledons, plasma membrane, proton symport, sucrose.


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