Journal of Experimental Botany, Vol. 51, No. 351, pp. 1687-1694,
October 2000
© 2000 Oxford University Press
Original Papers |
Rubisco activation state decreases with increasing nitrogen content in apple leaves
Department of Horticulture, Oregon State University, Corvallis, OR 97331, USA
Based on the curvilinear relationship between leaf nitrogen content and the initial slope of the response of CO2 assimilation (A) to intercellular CO2 concentrations (Ci) in apple, it is hypothesized that Rubisco activation state decreases with increasing leaf N content and this decreased activation state accounts for the curvilinear relationship between leaf N and CO2 assimilation. A range of leaf N content (1.0–5.0 g m-2) was achieved by fertilizing bench-grafted Fuji/M.26 apple (Malus domestica Borkh.) trees for 45 d with different N concentrations, using a modified Hoagland's solution. Analysis of A/Ci curves under saturating light indicated that CO2 assimilation at ambient CO2 fell within the Rubisco limitation region of the A/Ci curves, regardless of leaf N status. Initial Rubisco activity showed a curvilinear response to leaf N. In contrast, total Rubisco activity increased linearly with increasing leaf N throughout the leaf N range. As a result, Rubisco activation state decreased with increasing leaf N. Both light-saturated CO2 assimilation at ambient CO2 and the initial slope of the A/Ci curves were linearly related to initial Rubisco activity, but curvilinearly related to total Rubisco activity. The curvatures in the relationships of both light-saturated CO2 assimilation at ambient CO2 and the initial slope of the A/Ci curves with total Rubisco activity were more pronounced than in their relationships with leaf N. This was because the ratio of total Rubisco activity to leaf N increased with increasing leaf N. As leaf N increased, photosynthetic N use efficiency declined with decreasing Rubisco activation state.
Key words: Apple, CO2 assimilation, leaf N content, Malus domestica, Rubisco activation state.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. D. Jimenez, M. Pardos, J. Puertolas, L. A. Kleczkowski, and J. A. Pardos Deep shade alters the acclimation response to moderate water stress in Quercus suber L. Forestry, July 1, 2009; 82(3): 285 - 298. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Li, Y. Gao, X. Xu, Q. Shen, and S. Guo Light-saturated photosynthetic rate in high-nitrogen rice (Oryza sativa L.) leaves is related to chloroplastic CO2 concentration J. Exp. Bot., May 1, 2009; 60(8): 2351 - 2360. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Xia, L. Cheng, A. Lakso, and M. Goffinet Effects of Nitrogen Supply on Source-sink Balance and Fruit Size of 'Gala' Apple Trees J. Amer. Soc. Hort. Sci., January 1, 2009; 134(1): 126 - 133. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. K. Manter and J. Kerrigan A/Ci curve analysis across a range of woody plant species: influence of regression analysis parameters and mesophyll conductance J. Exp. Bot., December 1, 2004; 55(408): 2581 - 2588. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-S. Chen and L. Cheng Both xanthophyll cycle-dependent thermal dissipation and the antioxidant system are up-regulated in grape (Vitis labrusca L. cv. Concord) leaves in response to N limitation J. Exp. Bot., September 1, 2003; 54(390): 2165 - 2175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheng Xanthophyll cycle pool size and composition in relation to the nitrogen content of apple leaves J. Exp. Bot., January 2, 2003; 54(381): 385 - 393. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Cheng, L. H. Fuchigami, and P. J. Breen The relationship between photosystem II efficiency and quantum yield for CO2 assimilation is not affected by nitrogen content in apple leaves J. Exp. Bot., September 1, 2001; 52(362): 1865 - 1872. [Abstract] [Full Text] [PDF]
|
|