Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (23)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Scheenen, T.W.J.
Right arrow Articles by Van As, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Scheenen, T.W.J.
Right arrow Articles by Van As, H.
Agricola
Right arrow Articles by Scheenen, T.W.J.
Right arrow Articles by Van As, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 51, No. 351, pp. 1751-1759, October 2000
© 2000 Oxford University Press

Quantification of water transport in plants with NMR imaging

T.W.J. Scheenen, D. van Dusschoten1, P.A. de Jager and H. Van As2

Department of Biomolecular Sciences, Laboratory of Molecular Physics, Wageningen University, Dreijenlaan 3, 6703 HA, Wageningen, The Netherlands

A new nuclear magnetic resonance imaging (NMRi) method is described to calculate the characteristics of water transport in plant stems. Here, dynamic NMRi is used as a non-invasive technique to record the distribution of displacements of protons for each pixel in the NMR image. Using the NMR-signal of the stationary water in a reference tube for calibration, the following characteristics can be calculated per pixel without advance knowledge of the flow-profile in that pixel: the amount of stationary water, the amount of flowing water, the cross-sectional area of flow, the average linear flow velocity of the flowing water, and the volume flow. The accuracy of the method is demonstrated with a stem segment of a chrysanthemum flower by comparing the volume flow, measured with NMR, with the actual volumetric uptake, measured with a balance. NMR measurements corresponded to the balance uptake measurements with a rms error of 0.11 mg s-1 in a range of 0 to 1.8 mg s-1. Local changes in flow characteristics of individual voxels of a sample (e.g. intact plant) can be studied as a function of time and of any conceivable changes the sample experiences on a time-scale, longer than the measurement time of a complete set of pixel-propagators (17 min).

Key words: Water transport, flow quantification, xylem, NMR imaging, propagator.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
C. W. Windt, E. Gerkema, and H. Van As
Most Water in the Tomato Truss Is Imported through the Xylem, Not the Phloem: A Nuclear Magnetic Resonance Flow Imaging Study
Plant Physiology, October 1, 2009; 151(2): 830 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Capitani, F. Brilli, L. Mannina, N. Proietti, and F. Loreto
In Situ Investigation of Leaf Water Status by Portable Unilateral Nuclear Magnetic Resonance
Plant Physiology, April 1, 2009; 149(4): 1638 - 1647.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
T.W.J. Scheenen, F.J. Vergeldt, A.M. Heemskerk, and H. Van As
Intact Plant Magnetic Resonance Imaging to Study Dynamics in Long-Distance Sap Flow and Flow-Conducting Surface Area
Plant Physiology, June 1, 2007; 144(2): 1157 - 1165.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
H. Van As
Intact plant MRI for the study of cell water relations, membrane permeability, cell-to-cell and long distance water transport
J. Exp. Bot., March 1, 2007; 58(4): 743 - 756.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. van der Weerd, M. M.A.E. Claessens, T. Ruttink, F. J. Vergeldt, T. J. Schaafsma, and H. Van As
Quantitative NMR microscopy of osmotic stress responses in maize and pearl millet
J. Exp. Bot., December 1, 2001; 52(365): 2333 - 2343.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. Kockenberger
Nuclear magnetic resonance micro-imaging in the investigation of plant cell metabolism
J. Exp. Bot., April 1, 2001; 52(356): 641 - 652.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.