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Journal of Experimental Botany, Vol. 51, No. 352, pp. 1799-1811, November 1, 2000
© 2000 Oxford University Press

Original Papers

The fungal elicitor cryptogein induces cell wall modifications on tobacco cell suspension

Flore Kieffer1,5, Jeannine Lherminier2,5, Françoise Simon-Plas1,6, Michel Nicole3, Michel Paynot4, Taline Elmayan1 and Jean-Pierre Blein1

1 UMR 692, INRA-Université de Bourgogne, Laboratoire de Phytopharmacie et Biochimie des Interactions Cellulaires, BV 1540, 21034 Dijon cedex, France
2 INRA, Service Commun de Microscopie Electronique, BV 1540, 21034 Dijon cedex, France
3 IRD, GeneTrop Unité de Phytopathologie, BP 5045, 34032 Montpellier, France
4 Laboratoire de Phytoparasitologie, INRA/CNRS, Centre de Microbiologie du Sol et de l'Environnement, BV 1540, 21034 Dijon cedex, France

Upon addition of the fungal elicitor cryptogein, suspension cells of tobacco (Nicotiana tabacum cv. Xanthi) aggregated in clusters. Cytochemical experiments indicated that elicited cells displayed fibrillar expansions of pectin along the primary cell wall. Immunocytochemical detection of pectin epitopes indicated that the fibrillar material surrounding the treated cells was mostly composed of low methylated galacturonan sequences, but the use of the cationic probe did not reveal the presence of negatively charged carboxyl groups: the presence of important amounts of calcium ions in these pectic fibrillar expansions accounts for these observations. These data indicate that tobacco cells treated with cryptogein show a cell wall altered by the presence of a calcium pectate gel, resulting from the reorganization of pectin in the middle lamellae. These results are consistent with a drastic reduction in wall digestibility, partially reversed by increasing the pectolyase concentration in the hydrolytic solution. Diphenylene iodonium, an inhibitor of the oxidative burst triggered by cryptogein on tobacco cells, partially prevents elicited cell walls from this loss of digestibility, suggesting a possible role of active oxygen species in the cell wall strengthening. This work represents a new element of the signal transduction cascade triggered on tobacco cells by cryptogein.

Key words: Cell walls, cryptogein, pectic fibrillar expansions, Nicotiana tabacum, tobacco.


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