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Journal of Experimental Botany, Vol. 51, No. 353, pp. 1991-1999, December 2000
© 2000 Oxford University Press

Original Papers

Osmotic stress-induced changes of sucrose metabolism in cultured sweet potato cells

Heng-Long Wang1,2, Ping-Du Lee2, Wei-Liang Chen2, Dong-Jiann Huang2 and Jong-Ching Su1,2,3

1 Institute of Biological Chemistry, Academia Sinica, PO Box 23-106, Taipei, Taiwan 107, Republic of China
2 Biochemistry Laboratory, Department of Agricultural Chemistry, National Taiwan University, 1 Roosevelt Road Section 4, Taipei, Taiwan 107, Republic of China

The intra- and extracellular sugar contents, the activities of sucrose-metabolizing enzymes, and the metabolism of [U-14C] glucose in a pulse-chase experiment were compared between the normal and osmotically stressed (by 0.6 M sorbitol) sweet potato (Ipomoea batatas) suspension cells. The stress enhanced the levels of sucrose and sucrose phosphate synthase (SPS) activity. Northern blot analysis also showed that prolonged osmotic stress enhanced the SPS gene expression at the transcriptional level. Stressed cells also had higher activities of sucrose cleaving enzymes, such as alkaline invertase and sucrose synthase. The 14C-sucrose isolated from normal and stressed cells had 14C-fructose and 14C-glucose ratios of 0.68 and 1, respectively. These data suggest the continual cycling of degradation and synthesis of sucrose in both types of cells. Among the enzymes used in constructing such futile cycling, besides invertase and SPS, sucrose synthase (SS) should be involved in normal cells, but not in stressed ones. It is apparent that the osmotic stress caused a significant change in the pattern of sucrose metabolism.

Key words: Invertase, Ipomoea batatas, osmotic stress, radiotracer study, sucrose-phosphate synthase, sucrose synthase.


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