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Journal of Experimental Botany, Vol. 51, No. 353, pp. 2001-2007, December 2000
© 2000 Oxford University Press

Original Papers

Isolation and characterization of IDI2, a new Fe-deficiency-induced cDNA from barley roots, which encodes a protein related to the {alpha} subunit of eukaryotic initiation factor 2B (eIF2B{alpha})

Hirotaka Yamaguchi1,2, Hiromi Nakanishi1, Naoko K. Nishizawa3 and Satoshi Mori1,2,4

1 Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan
2 Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 4-1-8 Honcho, Kawaguchi-shi, 332-0012 Saitama, Japan
3 Laboratory of Plant Biotechnology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan

A new Fe-deficiency-inducible cDNA, IDI2, was isolated from Fe-deficient barley roots using the cDNA MACRO Array Technique. Accumulation of IDI2 transcripts in barley roots was strongly correlated with iron nutritional status. IDI2 encoded a protein with a low similarity to the {alpha} subunit of eukaryotic initiation factor 2B (eIF2B{alpha}). In addition, many hypothetical proteins homologous to IDI2 were also found in a database search. These proteins had limited similarity to eIF2B{alpha} as well as IDI2. It has been reported that these eIF2B{alpha}-like proteins (eIF2B{alpha}-LPs) are a family that is distinct from the eIF2B{alpha}/ß/{delta} family and widely distributed in the archaea, bacteria, and eukarya. A phylogenic analysis revealed that IDI2 is the first member of the eIF2B{alpha}-LP family to be found in higher plants. A possible role of IDI2 protein in regulating protein synthesis in Fe-deficient barley roots is proposed.

Key words: Barley, eIF2B, Fe-deficiency, iron, translation initiation factor.


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