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Journal of Experimental Botany, Vol. 52, No. 354, pp. 145-151, January 2001
© 2001 Oxford University Press

Original Papers

Heat shock-mediated APX gene expression and protection against chilling injury in rice seedlings

Yutaka Sato1,3, Toyotaka Murakami1, Hideyuki Funatsuki1, Shuichi Matsuba1, Haruo Saruyama2 and Masatoshi Tanida2

1 Hokkaido National Agricultural Experiment Station, Hitsujigaoka 1, Toyohira-ku, Sapporo 062-8555, Japan
2 Hokkaido Green-Bio Institute, Naganuma, Hokkaido 069-1301, Japan

Rice (Oryza sativa L.) seedlings, when kept at 42 °C for 24 h before being kept at 5 °C for 7 d, did not develop chilling injury. Chilling resistance was enhanced in parallel with the period of heat-treatment. The level of APX activity was higher in heated seedlings whereas CAT activity was decreased by heat stress. There was no significant difference in SOD activity between heated and unheated seedlings. The elevated activity of APX was sustained after 7 d of chilling. The cytosolic APX gene expression in response to high and low temperature was analysed with an APXa gene probe. APXa mRNA levels increased within 1 h after seedlings were exposed to 42 °C. Elevated APXa mRNA levels could also be detected after 24 h of heating. The APXa mRNA level in preheated seedlings was still higher than unheated seedlings under cold stress. The promoter of the APXa gene was cloned from rice genomic DNA by TAIL-PCR, and characterized by DNA sequencing. The promoter had a minimal heat shock factor-binding motif, 5'-nGAAnnTTCn-3', located in the 81 bp upstream of the TATA box. Heat shock induction of the APXa gene could be a possible cause of reduced chilling injury in rice seedlings.

Key words: Heat shock, chilling tolerance, ascorbate peroxidase, rice.


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