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Journal of Experimental Botany, Vol. 52, No. 354, pp. 181-182, January 2001
© 2001 Oxford University Press


Gene Notes

The AKT3 potassium channel protein interacts with the AtPP2CA protein phosphatase 2C

Eva Vranová1,3, Sari Tähtiharju2, Rutchadaporn Sriprang1, Hilde Willekens1,4, Pekka Heino2, E. Tapio Palva2, Dirk Inzé1,6 and Wim Van Camp1,5

1 Vakgroep Moleculaire Genetica and Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
2 Department of Biosciences, Division of Genetics, PO Box 56, FIN-00014 University of Helsinki, Finland

Abstract

The AKT3 potassium channel protein was identified as a strongly interacting partner of the Arabidopsis thaliana protein phosphatase 2C (AtPP2CA) in a yeast two-hybrid screen. A deletion analysis indicated that the catalytic domain of AtPP2CA was essential for the interaction with AKT3. Furthermore, the related PP2C phosphatase ABI1 did not interact with AKT3 in yeast.

Key words: Arabidopsis thaliana, potassium channel, PP2C, yeast two-hybrid screening.


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