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Journal of Experimental Botany, Vol. 52, No. 354, pp. 67-75, January 2001
© 2001 Oxford University Press

Original Papers

A ß-galactosidase-like gene is expressed during tobacco pollen development

Hilary Joan Rogers1, Sarah Louise Maund2 and Lisa Helen Johnson3

School of Biosciences, Cardiff University, PO Box 915, Cardiff CF10 3TL, UK

cDNA clone (TP5) with significant homology to ß-galactosidases has been isolated from a mature tobacco pollen cDNA library by differential screening. The predicted protein of 715 aa shows high levels of homology to plant ß-galactosidases expressed during fruit ripening and senescence. Northern analysis shows that the TP5 transcript is expressed exclusively in developing anthers and mature pollen. The transcript is present at very low levels at meiosis and increases dramatically, late in microspore development after mitosis suggesting that the primary role for the protein is during pollen tube growth. ß-galactosidase activity, measured by scanning densitometry of histochemically stained tobacco microspores, is first detectable in the early to mid-vacuolate stage, and reaches a peak at microspore mitosis, thereafter decreasing as the microspores reach maturity. Southern analysis indicates that the TP5 gene is present in two copies, probably corresponding to the two ancestral genomes of N. tabacum.

Key words: Pollen, microsporogenesis, ß-galactosidase, gene expression.


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