Journal of Experimental Botany, Vol. 52, No. 355, pp. 215-221,
February 2001
© 2001 Oxford University Press
Original Papers |
Auxin and heat shock activation of a novel member of the calmodulin like domain protein kinase gene family in cultured alfalfa cells
1 Institute of Plant Biology, Biological Research Center, Hungarian Academy of Sciences H-6701 Szeged, POB 521, Hungary
2 BAY ZOLTAN Foundation for Applied Research, Institute for Biotechnology H-6701 Szeged, POB 2337, Hungary
A calmodulin like domain protein kinase (CPK) homologue was identified in alfalfa and termed MsCPK3. The full-length sequence of cDNA encoded a 535 amino acid polypeptide with a molecular weight of 60.2 kDa. The deduced amino acid sequence showed all the conserved motifs that define other members of this kinase family, such as serine-threonine kinase domain, a junction region and four potential Ca2+-binding EF sites. The recombinant MsCPK3 protein purified from E. coli was activated by Ca2+and inhibited by calmodulin antagonist (W-7) in in vitro phosphorylation assays. The expression of MsCPK3 gene increased in the early phase of the 2,4-D induced alfalfa somatic embryogenesis. Heat shock also activated this gene while kinetin, ABA and NaCl treatment did not result in MsCPK3 mRNA accumulation. The data presented suggest that the new alfalfa CPK differs in stress responses from the previously described homologues and in its potential involvement in hormone and stress-activated reprogramming of developmental pathways during somatic embryogenesis.
Key words: Medicago sativa, CPK, stress, 2,4-D, phosphorylation, somatic embryogenesis.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M. L. Lanteri, G. C. Pagnussat, and L. Lamattina Calcium and calcium-dependent protein kinases are involved in nitric oxide- and auxin-induced adventitious root formation in cucumber J. Exp. Bot., March 1, 2006; 57(6): 1341 - 1351. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Szczegielniak, M. Klimecka, A. Liwosz, A. Ciesielski, S. Kaczanowski, G. Dobrowolska, A. C. Harmon, and G. Muszynska A Wound-Responsive and Phospholipid-Regulated Maize Calcium-Dependent Protein Kinase Plant Physiology, December 1, 2005; 139(4): 1970 - 1983. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Imin, M. Nizamidin, D. Daniher, K. E. Nolan, R. J. Rose, and B. G. Rolfe Proteomic Analysis of Somatic Embryogenesis in Medicago truncatula. Explant Cultures Grown under 6-Benzylaminopurine and 1-Naphthaleneacetic Acid Treatments Plant Physiology, April 1, 2005; 137(4): 1250 - 1260. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Leclercq, B. Ranty, M.-T. Sanchez-Ballesta, Z. Li, B. Jones, A. Jauneau, J.-C. Pech, A. Latche, R. Ranjeva, and M. Bouzayen Molecular and biochemical characterization of LeCRK1, a ripening-associated tomato CDPK-related kinase J. Exp. Bot., January 1, 2005; 56(409): 25 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. A. Ludwig, T. Romeis, and J. D. G. Jones CDPK-mediated signalling pathways: specificity and cross-talk J. Exp. Bot., January 2, 2004; 55(395): 181 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Hrabak, C. W.M. Chan, M. Gribskov, J. F. Harper, J. H. Choi, N. Halford, J. Kudla, S. Luan, H. G. Nimmo, M. R. Sussman, et al. The Arabidopsis CDPK-SnRK Superfamily of Protein Kinases Plant Physiology, June 1, 2003; 132(2): 666 - 680. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Charrier, A. Champion, Y. Henry, and M. Kreis Expression Profiling of the Whole Arabidopsis Shaggy-Like Kinase Multigene Family by Real-Time Reverse Transcriptase-Polymerase Chain Reaction Plant Physiology, October 1, 2002; 130(2): 577 - 590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. P. Pasternak, E. Prinsen, F. Ayaydin, P. Miskolczi, G. Potters, H. Asard, H. A. Van Onckelen, D. Dudits, and A. Feher The Role of Auxin, pH, and Stress in the Activation of Embryogenic Cell Division in Leaf Protoplast-Derived Cells of Alfalfa Plant Physiology, August 1, 2002; 129(4): 1807 - 1819. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Cheng, M. R. Willmann, H.-C. Chen, and J. Sheen Calcium Signaling through Protein Kinases. The Arabidopsis Calcium-Dependent Protein Kinase Gene Family Plant Physiology, June 1, 2002; 129(2): 469 - 485. [Abstract] [Full Text] [PDF]
|
|