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Journal of Experimental Botany, Vol. 52, No. 355, pp. 223-229, February 2001
© 2001 Oxford University Press

Original Papers

Ca2+ and phosphate releases from calcified Chara cell walls in concentrated KCl solution

Keitaro Kiyosawa1

Division of Biophysical Engineering, Graduate School of Engineering Science, Osaka University, Toyonaka, Osaka 560-8531, Japan

Ca2+ and Pi (inorganic phosphate) releases from isolated calcified and uncalcified Chara cell walls were measured with a Ca2+-selective electrode and colorimetry, and their ionic relations were analysed on the basis of the electroneutrality rule. The results showed that (1) not only Ca2+ but also Pi can be released from isolated calcified Chara cell walls into pure deionized water and 100 mM KCl solution, and (2) the positive charge due to the Ca2+ released cannot be neutralized only by the negative charge from the simultaneously released Pi. These findings suggest that calcium bands of calcified Chara cell walls are composed of mainly CaCO3 and CaHPO4 and some anions other than Pi should be released simultaneously with the Ca2+ and Pi. More Ca2+ and Pi can be solubilized from isolated Chara cell walls in 100 mM KCl solution than in pure deionized water. The pH value of 100 mM KCl solution in which isolated uncalcified young Chara cell walls have been immersed is a little lower than that of pure deionized water in which the same isolated uncalcified young Chara cell walls have been immersed, suggesting that some acidic substances are solubilized by 100 mM KCl. To explain this from the viewpoint of solution chemistry, the solubilities of pure CaCO3 and pure CaHPO4 in water and 100 mM KCl solution were measured with a Ca2+-selective electrode and their pH values with a glass pH electrode. The conclusion reached was that the Ca2+ release from isolated Chara cell walls is accompanied by the release of Pi, CO2-3 and acidic substances. This suggests that the so-called calcium bands and/or ionic relations, including ion exchange, in Chara cell walls are chemically or physicochemically more complex than they are currently considered to be.

Key words: Calcium band, calcium carbonate (CaCO3), calcium hydrogenphosphate (CaHPO4), cell wall, Chara corallina, electroneutrality rule.


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[Abstract] [Full Text] [PDF]


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