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Journal of Experimental Botany, Vol. 52, No. 358, pp. 1029-1040, May 1, 2001
© 2001 Oxford University Press

Original Papers

Changes in starch content in oat (Avena sativa) shoot pulvini during the gravitropic response

Soo-Chul Chang1, Myeong Haeng Cho2, Bin Goo Kang2 and Peter B. Kaufman1,3

1 Molecular, Cellular and Developmental Biology Group, Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048, USA
2 Department of Biology, Yonsei University, Seoul 120 749, Korea

In order to determine if components of the signal transduction pathway are involved in starch metabolism during the gravitropic response, the effects of inhibitors of phosphoprotein phosphatases and protein kinases (OA), and calcium channel blockers (LaCl3), on gravitropic bending and starch levels in gravisensitive node/pulvini of oat shoots were examined. Among the compounds tested, okadaic acid (OA) and lanthanum chloride (LaCl3) showed the strongest inhibitory effects on the negative gravitropic curvature response in oat shoot node/pulvini. At the same time, they caused a rapid loss of starch in graviresponding pulvini based on a quantitative analysis of starch levels in the bending tissues over 48 h periods. These two compounds act initially to block the net increase in starch content that occurs during the early stages (0–9 h) in graviresponding oat shoot pulvini. As a result, starch levels drop precipitously in shoots treated with OA and LaCl3, starting at time zero of gravistimulation by reorientation. These findings suggest that protein dephosphorylation and calcium play a role in starch metabolism in oat shoot pulvini in response to a gravistimulation signal. They also indicate that the amount of starch present in the chloroplast gravisensors in oat shoot pulvini may determine the rate of upward bending in graviresponding pulvini.

Key words: Calcium, gravitropism, protein dephosphorylation, oat, pulvini, starch metabolism.


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