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Journal of Experimental Botany, Vol. 52, No. 359, pp. 1173-1177, June 1, 2001
© 2001 Oxford University Press

Original Papers

Through pore diameter in the cell wall of Chara corallina

Genrikh N. Berestovsky1, Vadim I. Ternovsky and Anatolii A. Kataev

Institute of Cell Biophysics RAS, Pushchino, Moscow Region 142290, Russia

Determination of pore size of the cell wall of Chara corallina has been made by using the polyethylene glycol (PEG) series as the hydrophilic probing molecules. In these experiments, the polydispersity of commercial preparation of PEGs was allowed for. The mass share ({gamma}p) of polyethylene glycol preparation fractions penetrating through the pores was determined using a cellular ‘ghost’, i.e. fragments of internodal cell walls filled with a 25% solution of non-penetrating PEG 6000 and tied up at the ends. In water, such a ‘ghost’ developed a hydrostatic pressure close to the cell turgor which persisted for several days. The determination of {gamma}p, for polydisperse polyethylene glycols with different average molecular mass (M-) was calculated from the degree of pressure restoration after water was replaced by a 5–10% polymer solution. Pressure was recorded using a dynamometer, which measures, in the quasi-isometric mode, the force necessary for the partial compression of the ‘ghost’ in its small fragment. By utilizing the data on the distribution of PEG l000, 1450, 2000, and 3350 fractions over molecular mass (M), it was found that {gamma}p, for these polyethylene glycols corresponded to the upper limit of ML=800–1100 D (hydrodynamic radius of molecules, rh=0.85–1.05 nm). Thus, the effective diameter of the pores in the cell wall of Chara did not exceed 2.1 nm.

Key words: Chara, cell wall, pore size, polyethylene glycol, pressure relaxation.


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