Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (17)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Richard, C.
Right arrow Articles by Veylder, L. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Richard, C.
Right arrow Articles by Veylder, L. D.
Agricola
Right arrow Articles by Richard, C.
Right arrow Articles by Veylder, L. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 52, No. 361, pp. 1625-1633, August 1, 2001
© 2001 Oxford University Press

Original Papers

Analysis of cell division parameters and cell cycle gene expression during the cultivation of Arabidopsis thaliana cell suspensions

Caroline Richard1, Christine Granier2, Dirk Inzé1,3 and Lieven De Veylder1

1 Vakgroep Moleculaire Genetica, Departement Plantengenetica, Vlaams Interuniversitair Instituut voor Biotechnologie (VIB), Universiteit Gent, K.L. Ledeganckstraat 35, B-9000 Gent, Belgium
2 Institut National de la Recherche Agronomique, Laboratoire d'Ecophysiologie des Plantes sous Stress Environnementaux, 2 Place Viala, F-34060 Montpellier, France

Arabidopsis thaliana cell suspension cultures were characterized for the first time in detail in terms of biomass accumulation, cell division rate and cell cycle phase durations. Subsequently, this model system was used to follow the transcription profile of key cell cycle genes during a complete cultivation cycle. According to the calculated changes in the relative division rate over time, the cell cycle genes could be classified into four groups based on their transcriptional expression pattern. These differential patterns of gene expression are discussed with respect to the putative roles of the different cell cycle genes in the division cycle. Analysis of protein levels showed that mRNA levels did not correlate with protein levels in all cases. Results obtained in other systems, such as BY-2 cell suspensions or plants, confirm that cell suspension cultures of A. thaliana are suitable for the analysis of cell cycle regulation.

Key words: Arabidopsis thaliana, cell cycle, gene expression.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Physiol.Home page
K. K. Mandadi, A. Misra, S. Ren, and T. D. McKnight
BT2, a BTB Protein, Mediates Multiple Responses to Nutrients, Stresses, and Hormones in Arabidopsis
Plant Physiology, August 1, 2009; 150(4): 1930 - 1939.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Cedzich, H. Stransky, B. Schulz, and W. B. Frommer
Characterization of Cytokinin and Adenine Transport in Arabidopsis Cell Cultures
Plant Physiology, December 1, 2008; 148(4): 1857 - 1867.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
L. Pauwels, K. Morreel, E. De Witte, F. Lammertyn, M. Van Montagu, W. Boerjan, D. Inze, and A. Goossens
Mapping methyl jasmonate-mediated transcriptional reprogramming of metabolism and cell cycle progression in cultured Arabidopsis cells
PNAS, January 29, 2008; 105(4): 1380 - 1385.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
T. Yoshizumi, Y. Tsumoto, T. Takiguchi, N. Nagata, Y. Y. Yamamoto, M. Kawashima, T. Ichikawa, M. Nakazawa, N. Yamamoto, and M. Matsui
INCREASED LEVEL OF POLYPLOIDY1, a Conserved Repressor of CYCLINA2 Transcription, Controls Endoreduplication in Arabidopsis
PLANT CELL, October 1, 2006; 18(10): 2452 - 2468.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Konishi and M. Sugiyama
A Novel Plant-Specific Family Gene, ROOT PRIMORDIUM DEFECTIVE 1, Is Required for the Maintenance of Active Cell Proliferation
Plant Physiology, February 1, 2006; 140(2): 591 - 602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Verkest, C. Weinl, D. Inze, L. De Veylder, and A. Schnittger
Switching the Cell Cycle. Kip-Related Proteins in Plant Cell Cycle Control
Plant Physiology, November 1, 2005; 139(3): 1099 - 1106.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
K. Bisova, D. M. Krylov, and J. G. Umen
Genome-Wide Annotation and Expression Profiling of Cell Cycle Regulatory Genes in Chlamydomonas reinhardtii
Plant Physiology, February 1, 2005; 137(2): 475 - 491.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
G. Wang, H. Kong, Y. Sun, X. Zhang, W. Zhang, N. Altman, C. W. dePamphilis, and H. Ma
Genome-Wide Analysis of the Cyclin Family in Arabidopsis and Comparative Phylogenetic Analysis of Plant Cyclin-Like Proteins
Plant Physiology, June 1, 2004; 135(2): 1084 - 1099.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
N. BERTIN, M. GENARD, and S. FISHMAN
A Model for an Early Stage of Tomato Fruit Development: Cell Multiplication and Cessation of the Cell Proliferative Activity
Ann. Bot., July 1, 2003; 92(1): 65 - 72.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
K. Himanen, E. Boucheron, S. Vanneste, J. de Almeida Engler, D. Inze, and T. Beeckman
Auxin-Mediated Cell Cycle Activation during Early Lateral Root Initiation
PLANT CELL, October 1, 2002; 14(10): 2339 - 2351.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.