Journal of Experimental Botany, Vol. 52, No. 362, pp. 1817-1826,
September 1, 2001
© 2001 Oxford University Press
Original Papers |
Trehalose metabolism in Arabidopsis: occurrence of trehalose and molecular cloning and characterization of trehalose-6-phosphate synthase homologues
1 Botanisches Institut, Universität Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland
2 Max-Planck-Institute of Molecular Plant Physiology, D-14424 Potsdam, Germany
Axenically grown Arabidopsis thaliana plants were analysed for the occurrence of trehalose. Using gas chromatography–mass spectrometry (GC–MS) analysis, trehalose was unambiguously identified in extracts from Arabidopsis inflorescences. In a variety of organisms, the synthesis of trehalose is catalysed by trehalose-6-phosphate synthase (TPS; EC 2.4.1.15) and trehalose-6-phosphate phosphatase (TPP; EC 3.1.3.12). Based on EST (expressed sequence tag) sequences, three full-length Arabidopsis cDNAs whose predicted protein sequences show extensive homologies to known TPS and TPP proteins were amplified by RACE–PCR. The expression of the corresponding genes, AtTPSA, AtTPSB and AtTPSC, and of the previously described TPS gene, AtTPS1, was analysed by quantitative RT–PCR. All of the genes were expressed in the rosette leaves, stems and flowers of Arabidopsis plants and, to a lower extent, in the roots. To study the role of the Arabidopsis genes, the AtTPSA and AtTPSC cDNAs were expressed in Saccharomyces cerevisiae mutants deficient in trehalose synthesis. In contrast to AtTPS1, expression of AtTPSA and AtTPSC in the tps1 mutant lacking TPS activity did not complement trehalose formation after heat shock or growth on glucose. In addition, no TPP function could be identified for AtTPSA and AtTPSC in complementation studies with the S. cerevisiae tps2 mutant lacking TPP activity. The results indicate that while AtTPS1 is involved in the formation of trehalose in Arabidopsis, some of the Arabidopsis genes with homologies to known TPS/TPP genes encode proteins lacking catalytic activity in trehalose synthesis.
Key words: Arabidopsis, trehalose, trehalose-6-phosphate phosphatase, trehalose-6-phosphate synthase, yeast complementation.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Depuydt, S. Trenkamp, A. R. Fernie, S. Elftieh, J.-P. Renou, M. Vuylsteke, M. Holsters, and D. Vereecke An Integrated Genomics Approach to Define Niche Establishment by Rhodococcus fascians Plant Physiology, March 1, 2009; 149(3): 1366 - 1386. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. N. Chary, G. R. Hicks, Y. G. Choi, D. Carter, and N. V. Raikhel Trehalose-6-Phosphate Synthase/Phosphatase Regulates Cell Shape and Plant Architecture in Arabidopsis Plant Physiology, January 1, 2008; 146(1): 97 - 107. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Glinski and W. Weckwerth Differential Multisite Phosphorylation of the Trehalose-6-phosphate Synthase Gene Family in Arabidopsis thaliana: A Mass Spectrometry-based Process for Multiparallel Peptide Library Phosphorylation Analysis Mol. Cell. Proteomics, October 1, 2005; 4(10): 1614 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Avonce, B. Leyman, J. O. Mascorro-Gallardo, P. Van Dijck, J. M. Thevelein, and G. Iturriaga The Arabidopsis Trehalose-6-P Synthase AtTPS1 Gene Is a Regulator of Glucose, Abscisic Acid, and Stress Signaling Plant Physiology, November 1, 2004; 136(3): 3649 - 3659. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Q. Chen and G. G. Haddad Role of trehalose phosphate synthase and trehalose during hypoxia: from flies to mammals J. Exp. Biol., August 15, 2004; 207(18): 3125 - 3129. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J.H. v. Dijken, H. Schluepmann, and S. C.M. Smeekens Arabidopsis Trehalose-6-Phosphate Synthase 1 Is Essential for Normal Vegetative Growth and Transition to Flowering Plant Physiology, June 1, 2004; 135(2): 969 - 977. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Schluepmann, A. van Dijken, M. Aghdasi, B. Wobbes, M. Paul, and S. Smeekens Trehalose Mediated Growth Inhibition of Arabidopsis Seedlings Is Due to Trehalose-6-Phosphate Accumulation Plant Physiology, June 1, 2004; 135(2): 879 - 890. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Schluepmann, T. Pellny, A. van Dijken, S. Smeekens, and M. Paul Trehalose 6-phosphate is indispensable for carbohydrate utilization and growth in Arabidopsis thaliana PNAS, May 27, 2003; 100(11): 6849 - 6854. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. J. Eastmond, Y. Li, and I. A. Graham Is trehalose-6-phosphate a regulator of sugar metabolism in plants? J. Exp. Bot., January 3, 2003; 54(382): 533 - 537. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Paul and T. K. Pellny Carbon metabolite feedback regulation of leaf photosynthesis and development J. Exp. Bot., January 3, 2003; 54(382): 539 - 547. [Abstract] [Full Text] [PDF]
|
|