Journal of Experimental Botany, Vol. 52, No. 364, pp. 2097-2103,
November 1, 2001
© 2001 Oxford University Press
Original Papers |
Abscisic acid-specific binding sites in the flesh of developing apple fruit
Laboratory of Molecular Developmental Biology of Fruit Trees, Laboratory of Plant Physiology and Biochemistry, China Agricultural University, Beijing 100094, PR China
Abscisic acid (ABA) specific-binding sites localized in the cytosol were identified and characterized in the flesh of developing apple (Malus pumila L. cv. Starkrimon) fruit. ABA binding activity was scarcely detectable in the microsomes but high ABA binding activity in the cytosolic fraction was detected. The ABA-binding sites possessed a protein nature with both active serine residues and thiol-groups of cysteine residues in their functional binding sites. ABA binding was shown to be saturable, reversible and of high affinity. A Scatchard plot provided evidence for two different ABA binding proteins, one with higher affinity (Kd=2.3 nM) and the other with lower affinity (Kd=58.8 nM). Phaseic acid, trans-ABA and (-)-ABA had essentially no affinity for the binding proteins, indicating their stereo-specificity to bind physiologically active cis-(+)-ABA. The time-course, pH- and temperature-dependence of the ABA-binding proteins were determined. It is hypothesized that the detected ABA-binding proteins may be putative ABA-receptors that mediate ABA signals during fruit development.
Key words: Abscisic acid, ABA-binding protein, apple fruit.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Risk, C. L. Day, and R. C. Macknight Reevaluation of Abscisic Acid-Binding Assays Shows That G-Protein-Coupled Receptor2 Does Not Bind Abscisic Acid Plant Physiology, May 1, 2009; 150(1): 6 - 11. [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zhang, H. Ma, J. Feng, L. Zeng, Z. Wang, and S. Chen Grape berry plasma membrane proteome analysis and its differential expression during ripening J. Exp. Bot., August 1, 2008; 59(11): 2979 - 2990. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-F. Wang and D.-P. Zhang Abscisic Acid Receptors: Multiple Signal-perception Sites Ann. Bot., February 1, 2008; 101(3): 311 - 317. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Bruzzone, I. Moreschi, C. Usai, L. Guida, G. Damonte, A. Salis, S. Scarfi, E. Millo, A. De Flora, and E. Zocchi Abscisic acid is an endogenous cytokine in human granulocytes with cyclic ADP-ribose as second messenger PNAS, April 3, 2007; 104(14): 5759 - 5764. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X.-C. Yu, M.-J. Li, G.-F. Gao, H.-Z. Feng, X.-Q. Geng, C.-C. Peng, S.-Y. Zhu, X.-J. Wang, Y.-Y. Shen, and D.-P. Zhang Abscisic Acid Stimulates a Calcium-Dependent Protein Kinase in Grape Berry Plant Physiology, February 1, 2006; 140(2): 558 - 579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-P. Gao, Q.-H. Pan, M.-J. Li, L.-Y. Zhang, X.-F. Wang, Y.-Y. Shen, Y.-F. Lu, S.-W. Chen, Z. Liang, and D.-P. Zhang Abscisic Acid is Involved in the Water Stress-Induced Betaine Accumulation in Pear Leaves Plant Cell Physiol., June 15, 2004; 45(6): 742 - 750. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-Y. Zhang, Y.-B. Peng, S. Pelleschi-Travier, Y. Fan, Y.-F. Lu, Y.-M. Lu, X.-P. Gao, Y.-Y. Shen, S. Delrot, and D.-P. Zhang Evidence for Apoplasmic Phloem Unloading in Developing Apple Fruit Plant Physiology, May 1, 2004; 135(1): 574 - 586. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. A. Razem, M. Luo, J.-H. Liu, S. R. Abrams, and R. D. Hill Purification and Characterization of a Barley Aleurone Abscisic Acid-binding Protein J. Biol. Chem., March 12, 2004; 279(11): 9922 - 9929. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D.-P. Zhang, Z.-Y. Wu, X.-Y. Li, and Z.-X. Zhao Purification and Identification of a 42-Kilodalton Abscisic Acid-Specific-Binding Protein from Epidermis of Broad Bean Leaves Plant Physiology, February 1, 2002; 128(2): 714 - 725. [Abstract] [Full Text] [PDF]
|
|