Journal of Experimental Botany, Vol. 52, No. 364, pp. 2199-2206,
November 1, 2001
© 2001 Oxford University Press
Original Papers |
How the roots contribute to the ability of Phaseolus vulgaris L. to cope with chilling-induced water stress
1 Dipartimento di Biologia delle Piante Agrarie, Università degli Studi di Pisa, Viale delle Piagge 23, 56124 Pisa, Italy
2 Dipartimento di Scienze Agronomiche e Gestione del Territorio Agroforestale, Università degli Studi di Firenze, Piazzale delle Cascine 18, 50144 Firenze, Italy
3 Dipartimento di Produzione Vegetale, Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy
Intact plants and stem-girdled plants of Phaseolus vulgaris grown hydroponically were exposed to 5 °C for up to 4 d; stem girdling was used to inhibit the phloem transport from the leaves to the roots. After initial water stress, stomatal closure and an amelioration of root water transport properties allowed the plants to rehydrate and regain turgor. Chilling augmented the concentration of abscisic acid (ABA) content in leaves, roots and xylem sap. In intact plants stomatal closure and leaf ABA accumulation were preceded by a slight alkalinization of xylem sap, but they occurred earlier than any increase in xylem ABA concentration could be detected. Stem girdling did not affect the influence of chilling on plant water relations and leaf ABA content, but it reduced slightly the alkalinization of xylem sap and, principally, prevented the massive ABA accumulation in root tissues and the associated transport in the xylem that was observed in non-girdled plants. When the plants were defoliated just prior to chilling or after 10 h at 5 °C, root and xylem sap ABA concentration remained unchanged throughout the whole stress period. When the plants were chilled under conditions preventing the occurrence of leaf water deficit (i.e. at 100% relative humidity), there were no significant variations in endogenous ABA levels. The increase in root hydraulic conductance in chilled plants was a response neither to root ABA accretion, nor to some leaf-borne chemical signal transported downwards in the phloem, nor to low temperature per se, as indicated by the results of the experiments with defoliated or girdled plants and with plants chilled at 100% relative humidity. It was concluded that the root system contributed substantially to the bean's ability to cope with chilling-induced water stress, but not in an ABA-dependent manner.
Key words: Abscisic acid, acclimation, stomata, root hydraulic conductance, xylem sap pH.
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