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Journal of Experimental Botany, Vol. 52, No. 364, pp. 2239-2240, November 1, 2001
© 2001 Oxford University Press

Gene Notes

DNA replication in plants: characterization of a cdc6 homologue from Arabidopsis thaliana

G.B.A. Ramos1, J. de Almeida Engler2, P.C.G. Ferreira1 and A.S. Hemerly1,3

1 Departamento de Bioquímica Médica, ICB, Universidade Federal do Rio de Janeiro, 21941-590, Rio de Janeiro, RJ, Brazil
2 Vakgroep Moleculaire Genética, Department Plantengenetica, VIB, Universiteit Gent, B-9000 Gent, Belgium

Abstract

Cdc6 is a key regulator of DNA replication in eukaryotes. In this work, the expression pattern of an Arabidopsis cdc6 homologue is characterized by RT-PCR and in situ hybridization. The data suggest that cdc6At expression is cell cycle regulated. During development, high cdc6At mRNA levels are found in regular cycling cells. In addition, cdc6At expression is also observed in cells that are probably undergoing endoreduplication, suggesting a possible role of Cdc6At in this process in plants.

Key words: Arabidopsis thaliana, CDC6, DNA replication, pre-replication complex.


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