Journal of Experimental Botany, Vol. 53, No. 366, pp. 103-110,
January 1, 2002
© 2002 Oxford University Press
Original Papers |
Regulation of nitric oxide (NO) production by plant nitrate reductase in vivo and in vitro
1 Forschungszentrum Jülich GmbH, Institut für Biologie des Stoffaustauschs, 52425 Jülich, Germany
2 Forschungszentrum Jülich GmbH, Institut für Chemie der Belasteten Atmosphäre, 52425 Jülich, Germany
3 Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Molekulare Pflanzenphysiologie und Biophysik, Universität Würzburg, Julius-von-Sachs-Platz 2, 97082 Würzburg, Germany
NO (nitric oxide) production from sunflower plants (Helianthus annuus L.), detached spinach leaves (Spinacia oleracea L.), desalted spinach leaf extracts or commercial maize (Zea mays L.) leaf nitrate reductase (NR, EC 1.6.6.1) was continuously followed as NO emission into the gas phase by chemiluminescence detection, and its response to post-translational NR modulation was examined in vitro and in vivo. NR (purified or in crude extracts) in vitro produced NO at saturating NADH and nitrite concentrations at about 1% of its nitrate reduction capacity. The Km for nitrite was relatively high (100 µM) compared to nitrite concentrations in illuminated leaves (10 µM). NO production was competitively inhibited by physiological nitrate concentrations (Ki=50 µM). Importantly, inactivation of NR in crude extracts by protein phosphorylation with MgATP in the presence of a protein phosphatase inhibitor also inhibited NO production. Nitrate-fertilized plants or leaves emitted NO into purified air. The NO emission was lower in the dark than in the light, but was generally only a small fraction of the total NR activity in the tissue (about 0.01–0.1%). In order to check for a modulation of NO production in vivo, NR was artificially activated by treatments such as anoxia, feeding uncouplers or AICAR (a cell permeant 5'-AMP analogue). Under all these conditions, leaves were accumulating nitrite to concentrations exceeding those in normal illuminated leaves up to 100-fold, and NO production was drastically increased especially in the dark. NO production by leaf extracts or intact leaves was unaffected by nitric oxide synthase inhibitors. It is concluded that in non-elicited leaves NO is produced in variable quantities by NR depending on the total NR activity, the NR activation state and the cytosolic nitrite and nitrate concentration.
Key words: Helianthus annuus, nitrate reductase, nitric oxide, protein phosphorylation, Spinacia oleracea.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  M.-G. Zhao, L. Chen, L.-L. Zhang, and W.-H. Zhang Nitric Reductase-Dependent Nitric Oxide Production Is Involved in Cold Acclimation and Freezing Tolerance in Arabidopsis Plant Physiology, October 1, 2009; 151(2): 755 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Rodriguez-Serrano, M. C. Romero-Puertas, D. M. Pazmino, P. S. Testillano, M. C. Risueno, L. A. del Rio, and L. M. Sandalio Cellular Response of Pea Plants to Cadmium Toxicity: Cross Talk between Reactive Oxygen Species, Nitric Oxide, and Calcium Plant Physiology, May 1, 2009; 150(1): 229 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. U. Igamberdiev and R. D. Hill Plant mitochondrial function during anaerobiosis Ann. Bot., January 1, 2009; 103(2): 259 - 268. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Moreau, G. I. Lee, Y. Wang, B. R. Crane, and D. F. Klessig AtNOS/AtNOA1 Is a Functional Arabidopsis thaliana cGTPase and Not a Nitric-oxide Synthase J. Biol. Chem., November 21, 2008; 283(47): 32957 - 32967. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. J. Corpas, M. Chaki, A. Fernandez-Ocana, R. Valderrama, J. M. Palma, A. Carreras, J. C. Begara-Morales, M. Airaki, L. A del Rio, and J. B. Barroso Metabolism of Reactive Nitrogen Species in Pea Plants Under Abiotic Stress Conditions Plant Cell Physiol., November 1, 2008; 49(11): 1711 - 1722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Seligman, E. E. Saviani, H. C. Oliveira, C. A. F. Pinto-Maglio, and I. Salgado Floral Transition and Nitric Oxide Emission During Flower Development in Arabidopsis thaliana is Affected in Nitrate Reductase-Deficient Plants Plant Cell Physiol., July 1, 2008; 49(7): 1112 - 1121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Gilberthorpe and R. K. Poole Nitric Oxide Homeostasis in Salmonella typhimurium: ROLES OF RESPIRATORY NITRATE REDUCTASE AND FLAVOHEMOGLOBIN J. Biol. Chem., April 25, 2008; 283(17): 11146 - 11154. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. N. Bouchard and H. Yamasaki Heat Stress Stimulates Nitric Oxide Production in Symbiodinium microadriaticum: A Possible Linkage between Nitric Oxide and the Coral Bleaching Phenomenon Plant Cell Physiol., April 1, 2008; 49(4): 641 - 652. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Besson-Bard, C. Courtois, A. Gauthier, J. Dahan, G. Dobrowolska, S. Jeandroz, A. Pugin, and D. Wendehenne Nitric Oxide in Plants: Production and Cross-talk with Ca2+ Signaling Mol Plant, March 1, 2008; 1(2): 218 - 228. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Vitecek, V. Reinohl, and R. L. Jones Measuring NO Production by Plant Tissues and Suspension Cultured Cells Mol Plant, March 1, 2008; 1(2): 270 - 284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Neill, J. Bright, R. Desikan, J. Hancock, J. Harrison, and I. Wilson Nitric oxide evolution and perception J. Exp. Bot., January 1, 2008; 59(1): 25 - 35. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Wang, X. Xing, and N. Crawford Nitrite Acts as a Transcriptome Signal at Micromolar Concentrations in Arabidopsis Roots Plant Physiology, December 1, 2007; 145(4): 1735 - 1745. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Sugiura, M. N. Georgescu, and M. Takahashi A Nitrite Transporter Associated with Nitrite Uptake by Higher Plant Chloroplasts Plant Cell Physiol., July 1, 2007; 48(7): 1022 - 1035. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Liu, R. Wu, Q. Wan, G. Xie, and Y. Bi Glucose-6-Phosphate Dehydrogenase Plays a Pivotal Role in Nitric Oxide-Involved Defense Against Oxidative Stress Under Salt Stress in Red Kidney Bean Roots Plant Cell Physiol., March 1, 2007; 48(3): 511 - 522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Rusterucci, M. C. Espunya, M. Diaz, M. Chabannes, and M. C. Martinez S-Nitrosoglutathione Reductase Affords Protection against Pathogens in Arabidopsis, Both Locally and Systemically Plant Physiology, March 1, 2007; 143(3): 1282 - 1292. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I.G.L. Libourel, P.M. van Bodegom, M.D. Fricker, and R.G. Ratcliffe Nitrite Reduces Cytoplasmic Acidosis under Anoxia Plant Physiology, December 1, 2006; 142(4): 1710 - 1717. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Jasid, M. Simontacchi, C. G. Bartoli, and S. Puntarulo Chloroplasts as a Nitric Oxide Cellular Source. Effect of Reactive Nitrogen Species on Chloroplastic Lipids and Proteins Plant Physiology, November 1, 2006; 142(3): 1246 - 1255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Planchet and W. M. Kaiser Nitric oxide (NO) detection by DAF fluorescence and chemiluminescence: a comparison using abiotic and biotic NO sources J. Exp. Bot., September 1, 2006; 57(12): 3043 - 3055. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yamamoto-Katou, S. Katou, H. Yoshioka, N. Doke, and K. Kawakita Nitrate Reductase is Responsible for Elicitin-induced Nitric Oxide Production in Nicotiana benthamiana Plant Cell Physiol., June 1, 2006; 47(6): 726 - 735. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B Barroso, F. J Corpas, A. Carreras, M. Rodriguez-Serrano, F. J Esteban, A. Fernandez-Ocana, M. Chaki, M. C Romero-Puertas, R. Valderrama, L. M Sandalio, et al. Localization of S-nitrosoglutathione and expression of S-nitrosoglutathione reductase in pea plants under cadmium stress J. Exp. Bot., May 1, 2006; 57(8): 1785 - 1793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. N. Tun, C. Santa-Catarina, T. Begum, V. Silveira, W. Handro, E. I. S. Floh, and G. F. E. Scherer Polyamines Induce Rapid Biosynthesis of Nitric Oxide (NO) in Arabidopsis thaliana Seedlings Plant Cell Physiol., March 1, 2006; 47(3): 346 - 354. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lindermayr, G. Saalbach, G. Bahnweg, and J. Durner Differential Inhibition of Arabidopsis Methionine Adenosyltransferases by Protein S-Nitrosylation J. Biol. Chem., February 17, 2006; 281(7): 4285 - 4291. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. M. Crawford Mechanisms for nitric oxide synthesis in plants J. Exp. Bot., February 1, 2006; 57(3): 471 - 478. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Stohr and S. Stremlau Formation and possible roles of nitric oxide in plant roots J. Exp. Bot., February 1, 2006; 57(3): 463 - 470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perazzolli, M. C. Romero-Puertas, and M. Delledonne Modulation of nitric oxide bioactivity by plant haemoglobins J. Exp. Bot., February 1, 2006; 57(3): 479 - 488. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A. J. Mur, T. L. W. Carver, and E. Prats NO way to live; the various roles of nitric oxide in plant-pathogen interactions J. Exp. Bot., February 1, 2006; 57(3): 489 - 505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F.-Q. Guo and N. M. Crawford Arabidopsis Nitric Oxide Synthase1 Is Targeted to Mitochondria and Protects against Oxidative Damage and Dark-Induced Senescence PLANT CELL, December 1, 2005; 17(12): 3436 - 3450. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. U. IGAMBERDIEV, K. BARON, N. MANAC'H-LITTLE, M. STOIMENOVA, and R. D. HILL The Haemoglobin/Nitric Oxide Cycle: Involvement in Flooding Stress and Effects on Hormone Signalling Ann. Bot., September 1, 2005; 96(4): 557 - 564. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. A.J. Mur, I. E. Santosa, L. J.J. Laarhoven, N. J. Holton, F. J.M. Harren, and A. R. Smith Laser Photoacoustic Detection Allows in Planta Detection of Nitric Oxide in Tobacco following Challenge with Avirulent and Virulent Pseudomonas syringae Pathovars Plant Physiology, July 1, 2005; 138(3): 1247 - 1258. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Ohwaki, M. Kawagishi-Kobayashi, K. Wakasa, S. Fujihara, and T. Yoneyama Induction of Class-1 Non-symbiotic Hemoglobin Genes by Nitrate, Nitrite and Nitric Oxide in Cultured Rice Cells Plant Cell Physiol., February 1, 2005; 46(2): 324 - 331. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. U. Igamberdiev and R. D. Hill Nitrate, NO and haemoglobin in plant adaptation to hypoxia: an alternative to classic fermentation pathways J. Exp. Bot., December 1, 2004; 55(408): 2473 - 2482. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Sokolovski and M. R. Blatt Nitric Oxide Block of Outward-Rectifying K+ Channels Indicates Direct Control by Protein Nitrosylation in Guard Cells Plant Physiology, December 1, 2004; 136(4): 4275 - 4284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Perazzolli, P. Dominici, M. C. Romero-Puertas, E. Zago, J. Zeier, M. Sonoda, C. Lamb, and M. Delledonne Arabidopsis Nonsymbiotic Hemoglobin AHb1 Modulates Nitric Oxide Bioactivity PLANT CELL, October 1, 2004; 16(10): 2785 - 2794. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. J. Corpas, J. B. Barroso, A. Carreras, M. Quiros, A. M. Leon, M. C. Romero-Puertas, F. J. Esteban, R. Valderrama, J. M. Palma, L. M. Sandalio, et al. Cellular and Subcellular Localization of Endogenous Nitric Oxide in Young and Senescent Pea Plants Plant Physiology, September 1, 2004; 136(1): 2722 - 2733. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Zeier, M. Delledonne, T. Mishina, E. Severi, M. Sonoda, and C. Lamb Genetic Elucidation of Nitric Oxide Signaling in Incompatible Plant-Pathogen Interactions Plant Physiology, September 1, 2004; 136(1): 2875 - 2886. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. F. Vanin, D. A. Svistunenko, V. D. Mikoyan, V. A. Serezhenkov, M. J. Fryer, N. R. Baker, and C. E. Cooper Endogenous Superoxide Production and the Nitrite/Nitrate Ratio Control the Concentration of Bioavailable Free Nitric Oxide in Leaves J. Biol. Chem., June 4, 2004; 279(23): 24100 - 24107. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Lillo, C. Meyer, U. S. Lea, F. Provan, and S. Oltedal Mechanism and importance of post-translational regulation of nitrate reductase J. Exp. Bot., June 1, 2004; 55(401): 1275 - 1282. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. C. Bethke, M. R. Badger, and R. L. Jones Apoplastic Synthesis of Nitric Oxide by Plant Tissues PLANT CELL, February 1, 2004; 16(2): 332 - 341. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Desikan, M.-K. Cheung, J. Bright, D. Henson, J. T. Hancock, and S. J. Neill ABA, hydrogen peroxide and nitric oxide signalling in stomatal guard cells J. Exp. Bot., January 2, 2004; 55(395): 205 - 212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Garcia-Mata, R. Gay, S. Sokolovski, A. Hills, L. Lamattina, and M. R. Blatt Nitric oxide regulates K+ and Cl- channels in guard cells through a subset of abscisic acid-evoked signaling pathways PNAS, September 16, 2003; 100(19): 11116 - 11121. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Loque, P. Tillard, A. Gojon, and M. Lepetit Gene Expression of the NO3- Transporter NRT1.1 and the Nitrate Reductase NIA1 Is Repressed in Arabidopsis Roots by NO2-, the Product of NO3- Reduction Plant Physiology, June 1, 2003; 132(2): 958 - 967. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Desikan, R. Griffiths, J. Hancock, and S. Neill A new role for an old enzyme: Nitrate reductase-mediated nitric oxide generation is required for abscisic acid-induced stomatal closure in Arabidopsisthaliana PNAS, December 10, 2002; 99(25): 16314 - 16318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. M. Kaiser, H. Weiner, A. Kandlbinder, C.-B. Tsai, P. Rockel, M. Sonoda, and E. Planchet Modulation of nitrate reductase: some new insights, an unusual case and a potentially important side reaction J. Exp. Bot., April 15, 2002; 53(370): 875 - 882. [Abstract] [Full Text] [PDF]
|
|