Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (10)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Chen, X.
Right arrow Articles by Wood, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, X.
Right arrow Articles by Wood, A. J.
Agricola
Right arrow Articles by Chen, X.
Right arrow Articles by Wood, A. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 53, No. 367, pp. 225-232, February 1, 2002
© 2002 Oxford University Press

Original Papers

Characterization of the V-type H(+)-ATPase in the resurrection plant Tortula ruralis: accumulation and polysomal recruitment of the proteolipid c subunit in response to salt-stress1

Xinbo Chen2, Triwitayakorn Kanokporn2, Qin Zeng2, Thea A. Wilkins3 and Andrew J. Wood2,4

2 Department of Plant Biology, Southern Illinois University-Carbondale, Carbondale, IL 62901-6509, USA
3 Department of Agronomy and Range Science, University of California, Davis, CA 95616-8515, USA

Tortula ruralis is an important experimental system for the study of plant vegetative desiccation tolerance. EST gene discovery efforts utilizing desiccated gametophytes have identified a cDNA Vac1 encoding a predicted polypeptide with significant similarity to the vacuolar H+-ATPase c subunit. VAC1, the 167 amino acid deduced polypeptide, has a predicted molecular mass of 16.9 kDa, and a predicted pI of 9.7. Phylogenetic analysis demonstrated that previously characterized proteolipid polypeptide sequences could be reproducibly grouped into two major clades and that VAC1 forms a discrete evolutionary group. RNA blot and Western blot hybridizations were used to analyse expression of Vac1 and accumulation of VAC1 in response to (1) desiccation and rehydration, (2) increased NaCl concentration, and (3) NaCl-shock. During a desiccation–rehydration cycle, Vac1 transcripts are expressed in both the total and polysomal RNA fractions in approximately equal amounts, and the steady-state transcript levels are unchanged. However, Vac1 transcript levels increased in response to both elevated NaCl concentration and NaCl-shock. There is a preferential accumulation of Vac1 transcripts within the polysomal RNA fraction in response to salt stress, and these data suggest that T. ruralis possesses a salinity-stress-dependent and desiccation-stress-independent mechanism for post-transcriptional gene control. Using a cotton anti-c subunit polyclonal antibody raised against the C-terminal domain, it was shown that the amount of Tortula 16 kDa proteolipid in the tonoplast protein fraction was unaffected by any stress treatment.

Key words: Desiccation, moss, salinity, V-ATPase.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
W. Tyagi, D. Rajagopal, S. L. Singla-Pareek, M. K. Reddy, and S. K. Sopory
Cloning and Regulation of a Stress-regulated Pennisetum glaucum Vacuolar ATPase c Gene and Characterization of its Promoter that is Expressed in Shoot Hairs and Floral Organs
Plant Cell Physiol., August 1, 2005; 46(8): 1411 - 1422.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
Q. Zeng, X. Chen, and A. J. Wood
Two early light-inducible protein (ELIP) cDNAs from the resurrection plant Tortula ruralis are differentially expressed in response to desiccation, rehydration, salinity, and high light
J. Exp. Bot., May 1, 2002; 53(371): 1197 - 1205.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.