Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (11)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Kim, Y.-C.
Right arrow Articles by Choi, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kim, Y.-C.
Right arrow Articles by Choi, D.
Agricola
Right arrow Articles by Kim, Y.-C.
Right arrow Articles by Choi, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 53, No. 367, pp. 383-385, February 1, 2002
© 2002 Oxford University Press

Gene Note

Pathogen-induced expression of cyclo-oxygenase homologue in hot pepper (Capsicum annuum cv. Pukang)1

Young-Cheol Kim2, So-Young Yi2, Hyung Gon Mang3, Young Sam Seo3, Woo Taek Kim3,4 and Doil Choi2

2 Plant Cell Biotechnology Laboratory, Korea Research Institute of Bioscience and Biotechnology, PO Box 115, Yusung, Taejon 305–600, Korea
3 Department of Biology, College of Science, Yonsei University, Seoul 120–749, Korea

Abstract

The hypersensitive reaction (HR) in plants is typified by a rapid and localized cell death at the site of pathogen infection. To understand better the molecular and cellular defence mechanism controlling HR, hot pepper leaves (Capsicum annuum cv. Pukang) were inoculated with the soybean pustule pathogen Xanthomonas campestris pv. glycine 8ra. By using the DD-PCR technique, a cDNA fragment was identified that exhibited a sequence similarity to the recently identified tobacco pathogen-induced oxygenase (PIOX) with homology to animal cyclo-oxygenase (COX). Subsequently, the full-length cDNA clone, pCa-COX1, encoding the COX homologue from the pathogen-inoculated hot pepper leaf cDNA library was isolated. The deduced amino acid sequence of Ca-COX1 shares 85.8% identity with tobacco PIOX and displays a significant degree of sequence identity (21.7–23.7%) with mammalian COXs. The expression of Ca-COX1 was markedly induced at 4–12 h after pathogen infection, while HR cell death on pepper leaves appeared at approximately 15 h post-inoculation. These results are consistent with the notion that the lipid-derived signalling pathway is involved in the initial response of hot pepper plants to pathogen infection.

Key words: Cyclo-oxygenase, hot pepper, hypersensitive reaction, pathogen infection, plant defence mechanism.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
M. Koszelak-Rosenblum, A. C. Krol, D. M. Simmons, C. C. Goulah, L. Wroblewski, and M. G. Malkowski
His-311 and Arg-559 Are Key Residues Involved in Fatty Acid Oxygenation in Pathogen-inducible Oxygenase
J. Biol. Chem., September 5, 2008; 283(36): 24962 - 24971.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Tirajoh, T. S. T. Aung, A. B. McKay, and A. L. Plant
Stress-responsive {alpha}-dioxygenase expression in tomato roots
J. Exp. Bot., February 1, 2005; 56(412): 713 - 723.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.