Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Shibuya, K.
Right arrow Articles by Satoh, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shibuya, K.
Right arrow Articles by Satoh, S.
Agricola
Right arrow Articles by Shibuya, K.
Right arrow Articles by Satoh, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 53, No. 368, pp. 399-406, March 1, 2002
© 2002 Oxford University Press

Original Papers

Comparison of mRNA levels of three ethylene receptors in senescing flowers of carnation (Dianthus caryophyllus L.)

Kenichi Shibuya1, Masayasu Nagata2, Natsu Tanikawa2, Toshihito Yoshioka1, Teruyoshi Hashiba1 and Shigeru Satoh1,3

1 Laboratory of Bio-adaptation, Graduate School of Agricultural Sciences, Tohoku University, Tsutsumidori-amamiyamachi 1-1, Aoba-ku, Sendai 981-8555, Japan
2 Laboratory of Postharvest Physiology, National Institute of Vegetable and Tea Science, Ano, Mie 514-2392, Japan

Three ethylene receptor genes, DC-ERS1, DC-ERS2 and DC-ETR1, were previously identified in carnation (Dianthus caryophyllus L.). Here, the presence of mRNAs for respective genes in flower tissues and their changes during flower senescence are investigated by Northern blot analysis. DC-ERS2 and DC-ETR1 mRNAs were present in considerable amounts in petals, ovaries and styles of the flower at the full-opening stage. In the petals the level of DC-ERS2 mRNA showed a decreasing trend toward the late stage of flower senescence, whereas it increased slightly in ovaries and was unchanged in styles throughout the senescence period. However, DC-ETR1 mRNA showed no or little changes in any of the tissues during senescence. Exogenously applied ethylene did not affect the levels of DC-ERS2 and DC-ETR1 mRNAs in petals. Ethylene production in the flowers was blocked by treatment with 1,1-dimethyl-4-(phenylsulphonyl)semicarbazide (DPSS), but the mRNA levels for DC-ERS2 and DC-ETR1 decreased in the petals. DC-ERS1 mRNA was not detected in any cases. These results indicate that DC-ERS2 and DC-ETR1 are ethylene receptor genes responsible for ethylene perception and that their expression is regulated in a tissue-specific manner and independently of ethylene in carnation flowers during senescence.

Key words: Carnation, Dianthus caryophyllus, ethylene perception, ethylene-receptor mRNAs, flower senescence, petal wilting.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
J. Xue, Y. Li, H. Tan, F. Yang, N. Ma, and J. Gao
Expression of ethylene biosynthetic and receptor genes in rose floral tissues during ethylene-enhanced flower opening
J. Exp. Bot., May 1, 2008; 59(8): 2161 - 2169.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
W. G. van Doorn and E. J. Woltering
Physiology and molecular biology of petal senescence
J. Exp. Bot., March 3, 2008; (2008) erm356v2.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
N. Ma, H. Tan, X. Liu, J. Xue, Y. Li, and J. Gao
Transcriptional regulation of ethylene receptor and CTR genes involved in ethylene-induced flower opening in cut rose (Rosa hybrida) cv. Samantha
J. Exp. Bot., August 1, 2006; 57(11): 2763 - 2773.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
M. Iordachescu and S. Verlinden
Transcriptional regulation of three EIN3-like genes of carnation (Dianthus caryophyllus L. cv. Improved White Sim) during flower development and upon wounding, pollination, and ethylene exposure
J. Exp. Bot., August 1, 2005; 56(418): 2011 - 2018.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
Y.-F. CHEN, N. ETHERIDGE, and G. E. SCHALLER
Ethylene Signal Transduction
Ann. Bot., May 1, 2005; 95(6): 901 - 915.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
C. P. Yau, L. Wang, M. Yu, S. Y. Zee, and W. K. Yip
Differential expression of three genes encoding an ethylene receptor in rice during development, and in response to indole-3-acetic acid and silver ions
J. Exp. Bot., March 1, 2004; 55(397): 547 - 556.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.