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Journal of Experimental Botany, Vol. 53, No. 368, pp. 455-463, March 1, 2002
© 2002 Oxford University Press

Original Papers

Lipid metabolism in the moss Rhytidiadelphus squarrosus (Hedw.) Warnst. from lead-contaminated and non-contaminated populations

Irina A. Guschina1 and John L. Harwood2,3

1 Institute of Ecology of the Volga River Basin RAS, Togliatti 445003, Russia
2 Cardiff School of Biosciences, Cardiff University, PO Box 911, Cardiff CF10 3US, UK

Lipid metabolism and the effect of Pb2+ and Cu2+ on this process was studied in the moss Rhytidiadelphus squarrosus collected from both a lead-contaminated and a non-contaminated site. Total radiolabelling of lipids from [1-14C]acetate was similar in both populations and Cu or Pb (1 µM, 10 µM) did not cause much alteration in acute exposure experiments. However, there were significant qualitative changes. Of the major labelled neutral lipid classes, samples from the lead-polluted site showed a decrease in labelling of triacylglycerols and an increase in wax esters. Acute lead exposure caused similar effects. Cu caused a decrease in the labelling of wax esters and an increase in diacylglycerols. These data suggest that heavy metals cause a change in carbon flux through the acylation reactions associated with the Kennedy pathway. R. squarrosus obtained from the Pb-contaminated site also showed changes in polar lipid labelling compared to the uncontaminated site. The labelling of phosphatidylcholine was more than halved and replaced by increased labelling of other zwitterionic lipids. The chloroplast glycerolipids were also increasingly labelled. Acute exposure to Pb, however, caused little alteration of labelling patterns within 24 h. R. squarrosus contains high levels of polyunsaturated fatty acids (PUFAs), but moss obtained from the Pb-polluted site had significantly less PUFAs containing three or more double bonds. Such samples, when incubated with [1-14C]acetate also showed decreased PUFA labelling. By contrast, acute exposure to Pb produced different results. These data provide a foundation for examining lipid metabolism in bryophytes and the effects of pollution in this important class of organism. The results also emphasize that acute and chronic exposure to heavy metals may produce different effects and that caution must be exercised in extrapolating data from one system to another.

Key words: Bryophytes, copper, fatty acids, lead, lipids, metabolism.


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