Journal of Experimental Botany, Vol. 53, No. 368, pp. 535-543,
March 1, 2002
© 2002 Oxford University Press
Original Papers |
Characteristics of cadmium uptake in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens
1 IACR-Rothamsted, Agriculture and the Environment Division, Harpenden, Hertfordshire AL5 2JQ, UK
2 CSIRO Land and Water, PMB 2, Glen Osmond, SA 5064, Australia
Uptake of Cd and Zn by intact seedlings of two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens was characterized using radioactive tracers. Uptake of Cd and Zn at 2 °C was assumed to represent mainly apoplastic binding in the roots, whereas the difference in uptake between 22 °C and 2 °C represented metabolically dependent influx. There was no significant difference between the two ecotypes in the apoplastic binding of Cd or Zn. Metabolically dependent uptake of Cd was 4.5-fold higher in the high Cd-accumulating ecotype, Ganges, than in the low Cd-accumulating ecotype, Prayon. By contrast, there was only a 1.5-fold difference in the Zn uptake between the two ecotypes. For the Ganges ecotype, Cd uptake could be described by Michaelis–Menten kinetics with a Vmax of 143 nmol g-1 root FW h-1 and a Km of 0.45 µM. Uptake of Cd by the Ganges ecotype was not inhibited by La, Zn, Cu, Co, Mn, Ni or Fe(II), and neither by increasing the Ca concentration. By contrast, addition of La, Zn or Mn, or increasing the Ca concentration in the uptake solution decreased Cd uptake by Prayon. Uptake of Ca was larger in Prayon than in Ganges. The results suggest that Cd uptake by the low Cd-accumulating ecotype (Prayon) may be mediated partly via Ca channels or transporters for Zn and Mn. By contrast, there may exist a highly selective Cd transport system in the root cell membranes of the high Cd-accumulating ecotype (Ganges) of T. caerulescens.
Key words: Cd, hyperaccumulation, Thlaspi caerulescens, uptake, Zn.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  S. Uraguchi, S. Mori, M. Kuramata, A. Kawasaki, T. Arao, and S. Ishikawa Root-to-shoot Cd translocation via the xylem is the major process determining shoot and grain cadmium accumulation in rice J. Exp. Bot., July 1, 2009; 60(9): 2677 - 2688. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. H. Hassinen, M. Tuomainen, S. Peraniemi, H. Schat, S. O. Karenlampi, and A. I. Tervahauta Metallothioneins 2 and 3 contribute to the metal-adapted phenotype but are not directly linked to Zn accumulation in the metal hyperaccumulator, Thlaspi caerulescens J. Exp. Bot., January 1, 2009; 60(1): 187 - 196. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.-l. Lu, S.-k. Tian, X.-e Yang, X.-c. Wang, P. Brown, T.-q. Li, and Z.-l. He Enhanced root-to-shoot translocation of cadmium in the hyperaccumulating ecotype of Sedum alfredii J. Exp. Bot., August 1, 2008; 59(11): 3203 - 3213. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. L. Chaney, J. S. Angle, C. L. Broadhurst, C. A. Peters, R. V. Tappero, and D. L. Sparks Improved Understanding of Hyperaccumulation Yields Commercial Phytoextraction and Phytomining Technologies J. Environ. Qual., August 31, 2007; 36(5): 1429 - 1443. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Plaza, K. L. Tearall, F.-J. Zhao, P. Buchner, S. P. McGrath, and M. J. Hawkesford Expression and functional analysis of metal transporter genes in two contrasting ecotypes of the hyperaccumulator Thlaspi caerulescens J. Exp. Bot., May 1, 2007; 58(7): 1717 - 1728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. E. Tsyganov, A. A. Belimov, A. Y. Borisov, V. I. Safronova, M. Georgi, K.-J. Dietz, and I. A. Tikhonovich A Chemically Induced New Pea (Pisum sativum) Mutant SGECdt with Increased Tolerance to, and Accumulation of, Cadmium Ann. Bot., February 1, 2007; 99(2): 227 - 237. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. J. Lehto, W. Davison, H. Zhang, and W. Tych Theoretical comparison of how soil processes affect uptake of metals by diffusive gradients in thinfilms and plants. J. Environ. Qual., September 1, 2006; 35(5): 1903 - 1913. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Uraguchi, I. Watanabe, A. Yoshitomi, M. Kiyono, and K. Kuno Characteristics of cadmium accumulation and tolerance in novel Cd-accumulating crops, Avena strigosa and Crotalaria juncea J. Exp. Bot., September 1, 2006; 57(12): 2955 - 2965. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Cosio, L. DeSantis, B. Frey, S. Diallo, and C. Keller Distribution of cadmium in leaves of Thlaspi caerulescens J. Exp. Bot., February 1, 2005; 56(412): 765 - 775. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Y. Chan and B. A. Hale Differential accumulation of Cd in durum wheat cultivars: uptake and retranslocation as sources of variation J. Exp. Bot., December 1, 2004; 55(408): 2571 - 2579. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Cosio, E. Martinoia, and C. Keller Hyperaccumulation of Cadmium and Zinc in Thlaspi caerulescens and Arabidopsis halleri at the Leaf Cellular Level Plant Physiology, February 1, 2004; 134(2): 716 - 725. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Ramesh, R. Shin, D. J. Eide, and D. P. Schachtman Differential Metal Selectivity and Gene Expression of Two Zinc Transporters from Rice Plant Physiology, September 1, 2003; 133(1): 126 - 134. [Abstract] [Full Text] [PDF]
|
|