Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (7)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Matsushita, Y.
Right arrow Articles by Nyunoya, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Matsushita, Y.
Right arrow Articles by Nyunoya, H.
Agricola
Right arrow Articles by Matsushita, Y.
Right arrow Articles by Nyunoya, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 53, No. 369, pp. 765-767, April 1, 2002
© 2002 Oxford University Press

Gene Note

Isolation of a cDNA for a nucleoside diphosphate kinase capable of phosphorylating the kinase domain of the self-incompatibility factor SRK of Brassica campestris

Yasuhiko Matsushita1, Tatsuya Suzuki1, Ryo Kubota1, Masako Mori1, Hiroko Shimosato1, Masao Watanabe2, Toshiaki Kayano3, Takeshi Nishio4 and Hiroshi Nyunoya1,5

1 Gene Research Center, Tokyo University of Agriculture and Technology, Fuchu-shi, Tokyo 183-8509, Japan
2 Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan
3 National Institute of Agrobiological Sciences, Tukuba 305-8602, Japan
4 Faculty of Agriculture, Tohoku University, Sendai, 981-8555, Japan

Abstract

SRK is a plant receptor kinase involved in the self-incompatibility system of Brassica species. During a cDNA screening for the phosphoproteins from a stigma expression library, a clone encoding the nucleoside diphosphate kinase III (Bc-NDPK III) was obtained. After in vitro phosphorylation assays with recombinant proteins, Bc-NDPK III contained mostly phosphoserine. By contrast, the kinase domain of SRK contained phosphoserine and phosphothreonine, both of which were significantly increased by the addition of Bc-NDPK III in the presence of an SRK inhibitor KN-62. The result suggested the possible involvement of Bc-NDPK III in the signal transduction pathway through SRK.

Key words: Brassica campestris, nucleoside diphosphate kinase, self-incompatibility factor, SRK.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
P. E. Verslues, G. Batelli, S. Grillo, F. Agius, Y.-S. Kim, J. Zhu, M. Agarwal, S. Katiyar-Agarwal, and J.-K. Zhu
Interaction of SOS2 with Nucleoside Diphosphate Kinase 2 and Catalases Reveals a Point of Connection between Salt Stress and H2O2 Signaling in Arabidopsis thaliana
Mol. Cell. Biol., November 15, 2007; 27(22): 7771 - 7780.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
S. Dorion, F. Dumas, and J. Rivoal
Autophosphorylation of Solanum chacoense cytosolic nucleoside diphosphate kinase on Ser117
J. Exp. Bot., December 1, 2006; 57(15): 4079 - 4088.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. P. Kemp and J. Doughty
Just how complex is the BrassicaS-receptor complex?
J. Exp. Bot., January 1, 2003; 54(380): 157 - 168.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.