Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Waterworth, W. M.
Right arrow Articles by Bray, C. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Waterworth, W. M.
Right arrow Articles by Bray, C. M.
Agricola
Right arrow Articles by Waterworth, W. M.
Right arrow Articles by Bray, C. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 53, No. 371, pp. 1005-1015, May 2002
© 2002 Oxford University Press

Original Papers

Characterization of Arabidopsis photolyase enzymes and analysis of their role in protection from ultraviolet-B radiation

Wanda M. Waterworth 1, Qing Jiang, Christopher E. West, M. Nikaido and Clifford M. Bray

School of Biological Sciences, University of Manchester, Oxford Road, Manchester M13 9PT, UK

DNA photolyases are enzymes which mediate the light-dependent repair (photoreactivation) of UV-induced damage products in DNA by direct reversal of base damage rather than via excision repair pathways. Arabidopsis thaliana contains two photolyases specific for photoreactivation of either cyclobutane pyrimidine dimers (CPDs) or pyrimidine (6-4)pyrimidones (6-4PPs), the two major UV-B-induced photoproducts in DNA. Reduced FADH and a reduced pterin were identified as cofactors of the native Arabidopsis CPD photolyase protein. This is the first report of the chromophore composition of any native class II CPD photolyase protein to our knowledge. CPD photolyase protein levels vary between tissues and with leaf age and are highest in flowers and leaves of 3–5-week-old Arabidopsis plants. White light or UV-B irradiation induces CPD photolyase expression in Arabidopsis tissues. This contrasts with the 6-4PP photolyase protein which is constitutively expressed and not regulated by either white or UV-B light. Arabidopsis CPD and 6-4PP photolyase enzymes can remove UV-B-induced photoproducts from DNA in planta even when plants are grown under enhanced levels of UV-B irradiation and at elevated temperatures although the rate of removal of CPDs is slower at high growth temperatures. These studies indicate that Arabidopsis possesses the photorepair capacity to respond effectively to increased UV-B-induced DNA damage under conditions predicted to be representative of increases in UV-B irradiation levels at the Earth's surface and global warming in the twenty-first century.

Key words: Arabidopsis thaliana, CPD photolyase, 6-4PP photolyase, UVB.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Plant CellHome page
P. Casati, M. Campi, F. Chu, N. Suzuki, D. Maltby, S. Guan, A. L. Burlingame, and V. Walbot
Histone Acetylation and Chromatin Remodeling Are Required for UV-B-Dependent Transcriptional Activation of Regulated Genes in Maize
PLANT CELL, April 1, 2008; 20(4): 827 - 842.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
M. Teranishi, K. Nakamura, H. Morioka, K. Yamamoto, and J. Hidema
The Native Cyclobutane Pyrimidine Dimer Photolyase of Rice Is Phosphorylated
Plant Physiology, April 1, 2008; 146(4): 1941 - 1951.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
N. S. Baliga, S. J. Bjork, R. Bonneau, M. Pan, C. Iloanusi, M. C.H. Kottemann, L. Hood, and J. DiRuggiero
Systems Level Insights Into the Stress Response to UV Radiation in the Halophilic Archaeon Halobacterium NRC-1
Genome Res., June 1, 2004; 14(6): 1025 - 1035.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
R. Ulm, A. Baumann, A. Oravecz, Z. Mate, E. Adam, E. J. Oakeley, E. Schafer, and F. Nagy
Genome-wide analysis of gene expression reveals function of the bZIP transcription factor HY5 in the UV-B response of Arabidopsis
PNAS, February 3, 2004; 101(5): 1397 - 1402.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. Frohnmeyer and D. Staiger
Ultraviolet-B Radiation-Mediated Responses in Plants. Balancing Damage and Protection
Plant Physiology, December 1, 2003; 133(4): 1420 - 1428.
[Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.