Journal of Experimental Botany, Vol. 53, No. 372, pp. 1273-1282,
May 15, 2002
© 2002 Oxford University Press
Original Papers |
Active oxygen and cell death in cereal aleurone cells
Department of Plant and Microbial Biology, University of California, Berkeley, CA 94720-3102, USA
The cereal aleurone layer is a secretory tissue whose function is regulated by gibberellic acid (GA) and abscisic acid (ABA). Aleurone cells lack functional chloroplasts, thus excluding photosynthesis as a source of active oxygen species (AOS) in cell death. Incubation of barley aleurone layers or protoplasts in GA initiated the cell death programme, but incubation in ABA delays programmed cell death (PCD). Light, especially blue and UV-A light, and H2O2 accelerate PCD of GA-treated aleurone cells, but ABA-treated aleurone cells are refractory to light and H2O2 and are not killed. It was shown that light elevated intracellular H2O2, and that the rise in H2O2 was greater in GA-treated cells compared to cells in ABA. Experiments with antioxidants show that PCD in aleurone is probably regulated by AOS. The sensitivity of GA-treated aleurone to light and H2O2 is a result of lowered amounts of enzymes that metabolize AOS. mRNAs encoding catalase, ascorbate peroxidase and superoxide dismutase are all reduced during 6–18 h of incubation in GA, but these mRNAs were present in higher amounts in cells incubated in ABA. The amounts of protein and enzyme activities encoded by these mRNAs were also dramatically reduced in GA-treated cells. Aleurone cells store and metabolize neutral lipids via the glyoxylate cycle in response to GA, and glyoxysomes are one potential source of AOS in the GA-treated cells. Mitochondria are another potential source of AOS in GA-treated cells. AOS generated by these organelles bring about membrane rupture and cell death.
Key words: Abscisic acid, active oxygen, aleurone, cell death, gibberellic acid.
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