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Journal of Experimental Botany, Vol. 53, No. 373, pp. 1521-1524, June 2002
© 2002 Oxford University Press

Short Communications

Cloning and characterization of a phospholipase C from the C4 plant Digitaria sanguinalis

Sylvie Coursol1, Jean-Noël Pierre, Jean Vidal2 and Jeanine Grisvard

Institut de Biotechnologie des Plantes, UMR 8618, Université Paris XI, Bâtiment 630, 91405 Orsay Cedex, France

Abstract

As a PLC activity was implicated in the light transduction pathway that controls C4 photosynthesis in Digitaria sanguinalis, a full length PLC cDNA (DsPLC2) was cloned. The proteins encoded by the two possible open reading frames were produced in Escherichia coli; they both harbour a PLC activity but with different response to Ca2+ concentration, and with different sensitivity to the PLC inhibitor U-73122.

Key words: Monocot plant, phosphoinositide-specific phospholipase C, recombinant protein, translation initiation.


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