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Journal of Experimental Botany, Vol. 53, No. 374, pp. 1575-1580, July 1, 2002
© 2002 Oxford University Press

Establishment of a reproducible tissue culture system for the induction of Arabidopsis somatic embryos

Received 13 November 2001; Accepted 20 March 2002

Miho Ikeda-Iwai1,, Shinobu Satoh and Hiroshi Kamada

Gene Research Center, Institute of Biological Sciences, University of Tsukuba, Tsukuba, Ibaraki 305-8572, Japan

Abbreviations: ABA, abscisic acid; 2,4-D, 2,4 dichlorophenoxyacetic acid.

Somatic embryogenesis is an example of totipotency and is used as a model system for studying embryogenesis. A reproducible tissue culture system was established for the large-scale induction of Arabidopsis somatic embryos. The method allows maintenance of high embryogenic competence over a one-year period. Using this tissue culture system, the expression of embryo-specific genes (ABI3, LEC1, FUS3) was detected in embryogenic cells and somatic embryos. Exogenous application of abscisic acid enhanced the expression of some late-embryogenesis-abundant (LEA) protein genes in somatic embryos. The experiments show that the method can be used to obtain sufficient amounts of embryogenic material for basic molecular analyses.

Key words: Key words: Arabidopsis thaliana, embryo-specific gene expression, somatic embryogenesis, zygotic embryos.


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