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Journal of Experimental Botany, Vol. 53, No. 374, pp. 1677-1682, July 1, 2002
© 2002 Oxford University Press

Cloning an iron-regulated metal transporter from rice

Received 21 September 2001; Accepted 20 March 2002

Naimatullah Bughio1, Hirotaka Yamaguchi1,3, Naoko K. Nishizawa2,3, Hiromi Nakanishi1 and Satoshi Mori4,1,3

1 Laboratory of Plant Molecular Physiology, Department of Applied Biological Chemistry, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan
2 Laboratory of Plant Biotechnology, Department of Global Agricultural Sciences, The University of Tokyo, 1-1 Yayoi, Bunkyo-ku, 113-8657 Tokyo, Japan
3 Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, 4-1-8 Honcho, Kawaguchi-shi, 332-0012, Saitama, Japan

4 To whom correspondence should be addressed. Fax: +81 3 5841 8009. E-mail: asmori{at}mail.ecc.u-tokyo.ac.jp

Rice cDNA and genomic libraries were screened in order to clone an Fe(II) transporter gene. A cDNA clone highly homologous to the Arabidopsis Fe(II) transporter gene IRT1 was isolated from Fe-deficient rice roots. The cDNA clone was named OsIRT1. A genomic clone corresponding to the cDNA was also obtained, sequenced and analysed. When expressed in yeast cells, OsIRT1 cDNA reversed the growth defects of the yeast iron-uptake mutant. Northern blot analysis revealed that OsIRT1 mRNA was predominantly expressed in roots and was induced by Fe- and Cu-deficiency. This suggests that OsIRT1 is a functional metal transporter for iron, and is actively engaged in Fe uptake from soils, especially under limiting conditions.

Key words: Key words: Copper, Fe(II) transporter, iron, IRT1, rice.


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