Journal of Experimental Botany, Vol. 53, No. 375, pp. 1747-1751,
August 1, 2002
© 2002 Oxford University Press
The role of gibberellins in embryo axis development
Received 4 March 2002; Accepted 29 March 2002
Plant Physiology Research Group, Department of Biological Sciences, 2500 University Drive NW, University of Calgary, Calgary, Alberta, Canada T2N IN4
Abbreviations: DAC, days after culture (for microspore-derived embryos); DAP, days after pollination; MDE(s), microspore-derived embryo(s); SNK, Student–Newman–Keuls; ZEs, zygotic embryo(s).
The role of gibberellins (GAs) during early embryo development was examined using microspore-derived embryos (MDEs) of Brassica napus. At the globular stage of development, 10 d after initial culture (DAC) when endogenous GA1 levels are increasing rapidly, a triazole, uniconazole, was used at 1, 33 and 100 µM to inhibit GA biosynthesis. Within this dose range there was no apparent effect of the inhibitor on embryo growth through to the early torpedo stage. However, by 25 DAC uniconazole-treated MDEs showed significantly reduced (50%) axis elongation. Addition of GA1 at 33 µM on 14 DAC to embryos pretreated with 1 µM uniconazole on 10 DAC prevented this reduction in axis length, giving axis elongation equivalent to untreated MDEs. Application of GA1 alone, however, did not significantly increase axis elongation. The reduced axis growth seen with uniconazole treatment was due to reduced cell elongation, but not cell number, and the co-applied GA1 thus prevented the uniconazole-induced reduction in cell length. The elongating axis of MDEs may thus be a useful tool for examining the role of GAs in cell elongation.
Key words: Key words: Brassica napus, embryo development, gibberellins, microspore-derived embryos (MDEs), uniconazole.
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