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Journal of Experimental Botany, Vol. 53, No. 375, pp. 1833-1836, August 1, 2002
© 2002 Oxford University Press

Molecular cloning and characterization of plant genes encoding novel peroxisomal molybdoenzymes of the sulphite oxidase family

Received 25 February 2002; Accepted 20 May 2002

Tatsuo Nakamura4,2, Christian Meyer3 and Hiroshi Sano2

2  Research and Education Center for Genetic Information, Nara Institute of Science and Technology, Nara 630-0101, Japan
3  Unité de Nutrition Azotée des Plantes, INRA, 78026 Versailles, France

Abbreviations: Moco, molybdenum cofactor; NR(s), nitrate reductase(s); SOX(s), sulphite oxidase(s); EST(s), expressed sequence tag(s); cyt, cytochrome; FAD, flavin adenine dinucleotide; GFP, green fluorescent protein.

The Arabidopsis AtMCP and rice OsMCP genes which encode proteins highly homologous to molybdoenzymes of the sulphite oxidase family were isolated and characterized. Both proteins seemed to possess only a molybdenum cofactor as the redox centre, unlike all the other eukaryotic molybdoenzymes. Putative MCP orthologues were identified in 17 plant species, indicating that Mo possess only a molybdenum cofactor as the redox centre, unlike all the other eukaryotic molybdoenzymes. Putative MCP orthologues were identified in 17 plant species, indicating that MCPs are widely distributed over the plant kingdom. An analysis using a green fluorescent protein fusion showed that AtMCP possesses a peroxisomal targeting signal at its C-terminus. Putative peroxisomal targeting signals were also found in all plant MCPs, suggesting the existence of a new redox pathway in this organelle.

Key words: Key words: Expressed sequence tag, green fluorescent protein, molybdenum cofactor, nitrate reductase, peroxisomal targeting signal, redox reaction.


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