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Journal of Experimental Botany, Vol. 53, No. 376, pp. 1867-1870, September 1, 2002
© 2002 Oxford University Press

Combined expression of S-VSP{alpha} in two different organelles enhances its accumulation and total lysine production in leaves of transgenic tobacco plants

Received 11 January 2002; Accepted 3 June 2002

Dana Guenoune1, Rachel Amir2, Hanna Badani1, Shmuel Wolf3 and Shmuel Galili4,1

1 Agronomy and Natural Resources Department, Agricultural Research Organization, The Volcani Center, POB 6, Bet Dagan 50250, Israel
2 Department of Plant Physiology, Migal Technological Center, Kiryat Shmona 12100, Israel
3 The Institute of Plant Sciences and Genetics in Agriculture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel

4 To whom correspondence should be addressed. Fax: +972 3 9669642. E-mail: vclidg{at}netvision.net.il

Soybean vegetative storage proteins (S-VSPs) accumulate to high levels in vacuoles of both wild types and heterologous plants. Here it is shown that directing S-VSP{alpha} to two different organelles—chloroplasts and vacuoles—in a single transgenic plant significantly increased its accumulation. Accumulation of S-VSP{alpha} in heterologous plants correlated with total soluble lysine. Using this approach with essential amino-acid-rich transgene proteins may lead to a breakthrough in improving plant nutritional quality.

Key words: Key words: Chloroplasts, gene expression, improved nutritional quality, soybean vegetative storage proteins, total lysine content, transgenic tobacco plants.


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