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Journal of Experimental Botany, Vol. 53, No. 376, pp. 1899-1907, September 1, 2002
© 2002 Oxford University Press

Isolation and characterization of the orchid cytokinin oxidase DSCKX1 promoter

Received 7 January 2002; Accepted 11 June 2002

Shu Hua Yang, Hao Yu and Chong Jin Goh1

Plant Growth and Development Laboratory, Department of Biological Sciences, Faculty of Science, National University of Singapore, 10 Kent Ridge Crescent, Singapore 119260, Republic of Singapore

Abbreviations: BA, benzylaminopurine; CaMV, cauliflower mosaic virus; GUS, ß-glucuronidase; iP, isopentenyl adenine; iPA, isopentenyl adenosine; MS, Murashige–Skoog; PLBs, protocorm-like-bodies; 5'-RACE, 5'-rapid amplification of cDNA ends; ZR, zeatin riboside.

The orchid DSCKX1 is a new member of the cytokinin oxidase gene family, which catalyses the degradation of cytokinins bearing unsaturated isoprenoid side chains. A 3.7 kb fragment upstream of the DSCKX1 coding region was isolated, sequenced and characterized by deletion analysis of DSCKX1::ß-glucuronidase gene fusions using transient orchid and stable Arabidopsis transformation systems. Functional analysis of 5' deletions defined the 5'-upstream region that directs the expression in distinct tissues. Regulatory elements affecting the cytokinin induction of the DSCKX1 gene have also been delineated

Key words: Key words: Cytokinin oxidase, orchid, regulation.


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