Journal of Experimental Botany, Vol. 53, No. 378, pp. 2159-2166,
November 1, 2002
© 2002 Oxford University Press
Plasma membrane lipids in the resurrection plant Ramonda serbica following dehydration and rehydration
Received 10 December 2001; Accepted 3 July 2002
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1 Dipartimento di Chimica e Biotecnologie Agrarie, Università di Pisa, Via del Borghetto, 80, 56124 Pisa, Italy
2 Institute of Botany, University of Belgrade, Takovska 43, 11000 Belgrade, Yugoslavia
Abbreviations: ASG, acylated steryl glycosides; FS, free sterols; PA, phosphatidic acid; PL, phospholipids; PC, phosphatidylcholine; PE, phosphatidylethanolamine; PI, phosphatidylinositol; PG, phosphatidylglycerol; PM, plasma membrane; RWC, relative water content; SG, steryl glycosides.
Plants of Ramonda serbica were dehydrated to 3.6% relative water content (RWC) by withholding water for 3 weeks, afterwards the plants were rehydrated for 1 week to 93.8% RWC. Plasma membranes were isolated from leaves using a two-phase aqueous polymer partition system. Compared with well-hydrated (control) leaves, dehydrated leaves suffered a reduction of about 75% in their plasma membrane lipid content, which returned to the control level following rewatering. Also the lipid to protein ratio decreased after dehydration, almost regaining the initial value after rehydration. Lipids extracted from the plasma membrane of fully-hydrated leaves were characterized by a high level of free sterols and a much lower level of phospholipids. Smaller amounts of cerebrosides, acylated steryl glycosides and steryl glycosides were also detected. The main phospholipids of control leaves were phosphatidylcholine and phosphatidylethanolamine, whereas sitosterol was the free sterol present in the highest amount. Following dehydration, leaf plasma membrane lipids showed a constant level of free sterols and a reduction in phospholipids compared with the well-hydrated leaves. Both phosphatidylcholine and phosphatidylethanolamine decreased following dehydration, their molar ratio remaining unchanged. Among free sterols, the remarkably high cholesterol level present in the control leaves (about 14 mol%) increased 2-fold as a result of dehydration. Dehydration caused a general decrease in the unsaturation level of individual phospholipids and total lipids as well. Upon rehydration the lipid composition of leaf plasma membranes restored very quickly approaching the levels of well-hydrated leaves.
Key words: Key words: Dehydration, lipids, plasma membrane, Ramonda serbica, resurrection plants.
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