Journal of Experimental Botany, Vol. 53, No. 378, pp. 2177-2184,
November 1, 2002
© 2002 Oxford University Press
The hydraulic conductance of the angiosperm leaf lamina: a comparison of three measurement methods
Received 28 January 2002; Accepted 27 June 2002
Harvard University, Biological Laboratories, 16 Divinity Avenue, Cambridge, MA 02138, USA
1 To whom correspondence should be addressed. Fax: +1 617 496 5854. E-mail: LSACK{at}oeb.harvard.edu
A comparison was made of three methods for measuring the leaf lamina hydraulic conductance (Klamina) for detached mature leaves of six woody temperate angiosperm species. The high-pressure method, the evaporative flux method and the vacuum pump method involve, respectively, pushing, evaporating and pulling water out of the lamina while determining the flow rate into the petiole and the water potential drop across the leaf. Tests were made of whether the high-pressure method and vacuum pump method measurements of Klamina on single leaves were affected by irradiance. In Quercus rubra, the high pressure method was sensitive to irradiance; Klamina measured under high irradiance (>1200 µmol m–2 s–1 photosynthetically active radiation) was 4.6–8.8 times larger than under ambient laboratory lighting (
6 µmol m–2 s–1 photosynthetically active radiation). By constrast, the vacuum pump method was theoretically expected to be insensitive to irradiance, and this expectation was confirmed in experiments on Hedera helix. When used in the ways recommended here, the three methods produced measurements that agreed typically within 10%. There were significant differences in species’ Klamina; values ranged from 1.24x10–4 kg s–1 m–2 MPa–1 for Acer saccharum to 2.89x10–4 kg s–1 m–2 MPa–1 for Vitis labrusca. Accurate, rapid determination of Klamina will allow testing of the links between Klamina, water-use, drought tolerance, and the enormous diversity of leaf form, structure and composition.
Key words: Key words: Angiosperms, evaporative flux method, high-pressure method, hydraulic conductance, leaves, vacuum pump method.
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