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Journal of Experimental Botany, Vol. 53, No. 378, pp. 2279-2280, November 1, 2002
© 2002 Oxford University Press

Cloning of a grapevine Botrytis-responsive gene that has homology to the tobacco hypersensitivity-related hsr203J

Received 25 March 2002; Accepted 9 July 2002

Annie Bézier, Bernard Lambert and Fabienne Baillieul2,

Laboratoire de Biologie et Physiologie Végétales, Université de Reims Champagne-Ardenne, Equipe de Biochimie et Biologie Moléculaire des Plantes, URVVC-EA 2069, UFR Sciences, Moulin de la Housse–BP 1039, F-51687 Reims Cedex 2, France

2 To whom the correspondence should be addressed. Fax: +33 3 26 91 32 68. E-mail: fabienne.baillieul{at}univ-reims.fr

A cDNA encoding a putative HSR203J-like protein (BIG8.1) was obtained from total RNA isolated from Botrytis cinerea-infected grapevine leaves using differential display and RACE techniques. Real time RT-PCR analysis confirmed that the level of mRNA corresponding to BIG8.1 increased in grapevine leaves during the infection progress by B. cinerea. No significant change in mRNA level was observed in leaves after UV exposure. This expression pattern suggests that BIG8.1 could be a HR-specific marker in grapevine like hsr203J in tobacco.

Key words: Key words: Botrytis cinerea, hypersensitive response, UV stress, Vitis vinifera.


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