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Journal of Experimental Botany, Vol. 53, No. 378, pp. 2283-2285, November 1, 2002
© 2002 Oxford University Press

Characterization of a strawberry cDNA clone homologous to calcium-dependent protein kinases that is expressed during fruit ripening and affected by low temperature

Received 19 March 2002; Accepted 9 July 2002

Immaculada Llop-Tous, Eva Domínguez-Puigjaner and Miquel Vendrell1,

Departmento de Fisiología y Biodiversidad Molecular, Instituto de Biología Molecular de Barcelona, CSIC, Jordi Girona 18-26, 08034 Barcelona, Spain

Abbreviation: CDPK: calcium-dependent protein kinase.

A full-length cDNA clone (FaCDPK1) encoding a calcium-dependent protein kinase (CDPK) has been isolated from a strawberry fruit cDNA library. FaCDPK1 contains the basic features of CDPKs: a catalytic kinase domain linked to a regulatory calmodulin-like domain by a junction sequence that has been shown to act as an autoinhibitory pseudosubstrate. Although the calmodulin-like domain of CDPKs typically contains four EF-hand calcium-binding motifs, FaCDPK1 was predicted to contain only three EF-hand motifs. FaCDPK1 gene expression was observed in roots, stolons, meristems, flowers, and leaves. FaCDPK1 mRNA was not detected in young fruits, but accumulated as fruit turned to white, suggesting a role for this gene in the developing strawberry fruit. In ripe fruit the levels of transcript increased in response to low temperature.

Key words: Key words: CDPK, cold treatment, strawberry, fruit.


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