Journal of Experimental Botany, Vol. 53, No. 379, pp. 2333-2339,
December 1, 2002
© 2002 Oxford University Press
Characterization of two putative ethylene receptor genes expressed during peach fruit development and abscission
Received 7 May 2002; Accepted 19 July 2002
Department of Environmental Agronomy and Crop Science, University of Padova, Via Romea, 16-Agripolis, Legnaro (Padova), 35020 Italy
1 To whom correspondence should be addressed. Fax: +39 049 8272850. e-mail: angelo.ramina{at}unipd.it
Abbreviations: 1-MCP, 1-methylcyclopropene, AZ, abscission zone; DNZ, distal non-zone; NZ, non-zone; PNZ, proximal non-zone.
Two peach genes homologous to the Arabidopsis ethylene receptor genes ETR1 and ERS1, named Pp-ETR1 and Pp-ERS1 respectively, have been isolated and characterized. Pp-ETR1 and Pp-ERS1 are conserved in terms of exon numbers and intron positions, although the first and fifth introns of Pp-ETR1 have an unusual length. In addition, two putative polyadenylation sites, that may cause an incomplete splicing at the 3' terminus, are present in the fifth intron. A motif of 28 nt, which shows high homology with ethylene responsive elements found in promoters of genes up-regulated by ethylene, is present in the promoter region of Pp-ERS1. Expression analysis, carried out by quantitative RT-PCR, was performed during fruit development and ripening, and leaf and fruitlet abscission. The level of Pp-ETR1 transcripts remained unchanged in all the tissues and developmental stages examined, whereas Pp-ERS1 mRNA abundance increased in ripening mesocarp, in leaf and fruitlet activated abscission zones, and following propylene application. 1-methylcyclopropene (1-MCP), an inhibitor of ethylene action, did not affect Pp-ETR1 transcription, while it down-regulated Pp-ERS1. A rise in ethylene evolution, accompanied by an increase of Pp-ERS1 transcript accumulation occurred within 24 h from the end of 1-MCP treatment. These results indicate that Pp-ERS1 might play a role in abscission and ripening.
Key words: Abscission zone, ERS1, ETR1, 1-methylcyclopropene (1-MCP), quantitative RT-PCR, ripening, Prunus persica.
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