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Journal of Experimental Botany, Vol. 53, No. 379, pp. 2451-2452, December 1, 2002
© 2002 Oxford University Press

Isolation and expression of a novel starch-storing cell-specific gene containing the KH RNA binding domain from tobacco-cultured cells BY-2

Received 29 July 2002; Accepted 21 August 2002

Yutaka Miyazawa5,1, Atsushi Sakai2, Sachihiro Matsunaga3, Tadao Asami1, Shigeyuki Kawano3, Tsuneyoshi Kuroiwa4 and Shigeo Yoshida1

1 Plant Functions Laboratory, RIKEN, Hirosawa, Wako-shi, Saitama, 351-0198, Japan
2 Department of Biological Sciences, Faculty of Science, Nara Women’s University, Nara 630-8506, Japan
3 Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo, Kashiwanoha, Kashiwa, Chiba 277-8562, Japan
4 Department of Biological Sciences, Graduate School of Science, University of Tokyo, Hongo, Tokyo 113-0033, Japan

5 To whom correspondence should be addressed. Fax: +81 48 462 4674. E-mail: yutakam{at}postman.riken.go.jp

In cultured Bright Yellow-2 tobacco (Nicotiana tabacum) cells, the depletion of 2,4-dichlorophenoxyacetic acid (2,4-D) in the culture medium induces amyloplast development. This differentiation also includes a decrease in cell multiplication, and an increase in cell size. These changes were primarily triggered by the depletion of 2,4-D, and accelerated by the addition of benzyladenine (BA). Three cDNAs were identified whose transcript levels are specifically increased during differentiation of starch-storing cells using the differential display method, and designated as starch-storing cell induced genes (SCI genes). One of these cDNAs, SCI2 encodes a 285 amino acids long protein with a KH RNA-binding domain. A database search revealed that this protein showed similarity to respective domains of mammalian quaking proteins. 2,4-D addition, which can convert starch-storing cells into dividing cells, to starch-storing BY-2 cells, immediately decreases the SCI2 transcript level, suggesting that SCI2 may have some role in starch-storing cell differentiation in BY-2 cells.

Key words: 2,4-D, benzyladenine, BY-2 cells, differentiation, starch-storing cells, tobacco.


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 J Exp BotHome page
Y. Miyazawa, N. Nakajima, T. Abe, A. Sakai, S. Fujioka, S. Kawano, T. Kuroiwa, and S. Yoshida
Activation of cell proliferation by brassinolide application in tobacco BY-2 cells: effects of brassinolide on cell multiplication, cell-cycle-related gene expression, and organellar DNA contents
J. Exp. Bot., December 1, 2003; 54(393): 2669 - 2678.
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