Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (6)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Hiscock, S. J.
Right arrow Articles by Brennan, A. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hiscock, S. J.
Right arrow Articles by Brennan, A. C.
Agricola
Right arrow Articles by Hiscock, S. J.
Right arrow Articles by Brennan, A. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 380, pp. 169-174, January 1, 2003
© 2003 Oxford University Press

Sporophytic self-incompatibility in Senecio squalidus L. (Asteraceae)—the search for S

Received 12 April 2002; Accepted 22 July 2002

Simon J. Hiscock3,1, Stephanie M. McInnis1, David A. Tabah1, Catherine A. Henderson2 and Adrian C. Brennan2

1 School of Biological Sciences, University of Bristol, Woodland Road, Clifton, Bristol BS8 1UG, UK
2 Department of Plant Sciences, South Parks Road, University of Oxford, Oxford OX1 3RB, UK

3 To whom correspondence should be addressed. Fax: +44 (0)117 9257374. E-mail: simon.hiscock{at}bristol.ac.uk

Senecio squalidus (Oxford Ragwort) is being used as a model species to study the genetics and molecular genetics of self-incompatibility (SI) in the Asteraceae. S. squalidus has a strong system of sporophytic SI (SSI) and populations within the UK contain very few S alleles probably due to a population bottleneck experienced on its introduction to the UK. The genetic control of SSI in S. squalidus is complex and may involve a second locus epistatic to S. Progress towards identifying the female determinant of SSI in S. squalidus is reviewed here. Research is focused on plants carrying two defined S alleles, S1 and S2. S2 is dominant to S1 in pollen and stigma. RT-PCR was used to amplify three SRK-like cDNAs from stigmas of S1S2 heterozygotes, but the expression patterns of these cDNAs suggest that they are unlikely to be directly involved in SI or pollen–stigma interactions in contrast to SSI in the Brassicaceae. Stigma-specific proteins associated with the S1 allele and the S2 allele have been identified using isoelectric focusing and these proteins have been designated SSP1 (Stigma S-associated Protein 1) and SSP2. SSP1 and SSP2 cDNAs have been cloned by 3' and 5' RACE and shown to be allelic forms of the same gene, SSP. The expression of SSP and its linkage to the S locus are currently being investigated. Initial results show SSP to be expressed exclusively in stigmas and developmentally regulated, with maximal expression occurring at and just before anthesis when SI is fully functional, SSP expression being undetectable in immature buds. Together these data suggest that SSP is a strong candidate for a Senecio S-gene.

Key words: Asteraceae, S-gene, Senecio squalidus, sporophytic self-incompatibility, SRK-like gene.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol Biol EvolHome page
N. L. Prigoda, A. Nassuth, and B. K. Mable
Phenotypic and Genotypic Expression of Self-incompatibility Haplotypes in Arabidopsis lyrata Suggests Unique Origin of Alleles in Different Dominance Classes
Mol. Biol. Evol., July 1, 2005; 22(7): 1609 - 1620.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.