Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (51)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Camacho, L.
Right arrow Articles by Malhó, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Camacho, L.
Right arrow Articles by Malhó, R.
Agricola
Right arrow Articles by Camacho, L.
Right arrow Articles by Malhó, R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 380, pp. 83-92, January 1, 2003
© 2003 Oxford University Press

Endo/exocytosis in the pollen tube apex is differentially regulated by Ca2+ and GTPases

Received 12 April 2002; Accepted 19 September 2002

Luísa Camacho and Rui Malhó1,

Departamento Biologia Vegetal, Faculdade de Ciências de Lisboa, 1749-017 Lisboa, Portugal

1 To whom correspondence should be addressed. Fax: +351 217500069. E-mail: r.malho{at}fc.ul.pt

Pollen tube growth relies on an extremely fast delivery of new membrane and wall material to the apical region where growth takes place. Despite the obvious meaning of this fact, the mechanisms that control this process remain very much unknown. It has previously been shown that apical growth is regulated by cytosolic free calcium ([Ca2+]c) so it was decided to test how changes in [Ca2+]c affect endo/exocytosis in pollen tube growth and reorientation. The endo/exocytosis was assayed in living cells using confocal imaging of FM 1-43. It was found that growing pollen tubes exhibited a higher endo/exocytosis activity in the apical region whereas in non-growing cells FM 1-43 is uniformly distributed. During pollen tube reorientation, a spatial redistribution of exocytotic activity was observed with the highest fluorescence in the side to which the cell will bend. Localized increases in [Ca2+]c induced by photolysis of caged Ca2+ increased exocytosis. In order to find if [Ca2+]c changes were modulating endo/exocytosis directly or through a signalling cascade, tests were conducted to find how changes in GTP levels and GTPase activity (primary regulators of the secretory pathway) affect the apical [Ca2+]c gradient and endo/exocytosis. It was found that increases in GTP levels could promote exocytosis (and growth). Interestingly, the increase in [GTP] did not significantly affect [Ca2+]c distribution, thus suggesting that the apical endo/exocytosis is regulated in a concerted but differentiated manner by the Ca2+ gradient and the activity of GTPases. Rop GTPases are likely candidates to mediate the Ca2+/GTP cross-talk as shown by knock-down experiments in growing pollen tubes.

Key words: Antisense, caged probes, calcium, endocytosis, exocytosis, GTP, pollen tubes, rop GTPase.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
G. Cai and M. Cresti
Organelle motility in the pollen tube: a tale of 20 years
J. Exp. Bot., February 1, 2009; 60(2): 495 - 508.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. B. Lee, S. Kim, and B. McClure
A Pollen Protein, NaPCCP, That Binds Pistil Arabinogalactan Proteins Also Binds Phosphatidylinositol 3-Phosphate and Associates with the Pollen Tube Endomembrane System
Plant Physiology, February 1, 2009; 149(2): 791 - 802.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
E. Sousa, B. Kost, and R. Malho
Arabidopsis Phosphatidylinositol-4-Monophosphate 5-Kinase 4 Regulates Pollen Tube Growth and Polarity by Modulating Membrane Recycling
PLANT CELL, November 1, 2008; 20(11): 3050 - 3064.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
Y. J. Lee, A. Szumlanski, E. Nielsen, and Z. Yang
Rho-GTPase-dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
J. Cell Biol., October 22, 2008; 181(7): 1155 - 1168.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. B. Lee, K. N. Swatek, and B. McClure
Pollen Proteins Bind to the C-terminal Domain of Nicotiana alata Pistil Arabinogalactan Proteins
J. Biol. Chem., October 3, 2008; 283(40): 26965 - 26973.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Bove, B. Vaillancourt, J. Kroeger, P. K. Hepler, P. W. Wiseman, and A. Geitmann
Magnitude and Direction of Vesicle Dynamics in Growing Pollen Tubes Using Spatiotemporal Image Correlation Spectroscopy and Fluorescence Recovery after Photobleaching
Plant Physiology, August 1, 2008; 147(4): 1646 - 1658.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Bot.Home page
L. L. Ge, H. Q. Tian, and S. D. Russell
Calcium function and distribution during fertilization in angiosperms
Am. J. Botany, June 1, 2007; 94(6): 1046 - 1060.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
T. J. Bushart and S. J. Roux
Conserved Features of Germination and Polarized Cell Growth: A Few Insights from a Pollen-Fern Spore Comparison
Ann. Bot., January 1, 2007; 99(1): 9 - 17.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
P. Campanoni and M. R. Blatt
Membrane trafficking and polar growth in root hairs and pollen tubes
J. Exp. Bot., January 1, 2007; 58(1): 65 - 74.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
A. Y. Cheung and H.-M. Wu
Structural and functional compartmentalization in pollen tubes
J. Exp. Bot., January 1, 2007; 58(1): 75 - 82.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
L. Cardenas, S. T. McKenna, J. G. Kunkel, and P. K. Hepler
NAD(P)H Oscillates in Pollen Tubes and Is Correlated with Tip Growth
Plant Physiology, December 1, 2006; 142(4): 1460 - 1468.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
G. M. Yoon, P. E. Dowd, S. Gilroy, and A. G. McCubbin
Calcium-Dependent Protein Kinase Isoforms in Petunia Have Distinct Functions in Pollen Tube Growth, Including Regulating Polarity
PLANT CELL, April 1, 2006; 18(4): 867 - 878.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Q. Wang, L. Kong, H. Hao, X. Wang, J. Lin, J. Samaj, and F. Baluska
Effects of Brefeldin A on Pollen Germination and Tube Growth. Antagonistic Effects on Endocytosis and Secretion
Plant Physiology, December 1, 2005; 139(4): 1692 - 1703.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
B. H.J. de Graaf, A. Y. Cheung, T. Andreyeva, K. Levasseur, M. Kieliszewski, and H.-m. Wu
Rab11 GTPase-Regulated Membrane Trafficking Is Crucial for Tip-Focused Pollen Tube Growth in Tobacco
PLANT CELL, September 1, 2005; 17(9): 2564 - 2579.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
L.-z. Tao, A. Y. Cheung, C. Nibau, and H.-m. Wu
RAC GTPases in Tobacco and Arabidopsis Mediate Auxin-Induced Formation of Proteolytically Active Nuclear Protein Bodies That Contain AUX/IAA Proteins
PLANT CELL, August 1, 2005; 17(8): 2369 - 2383.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
R. A. Cole, L. Synek, V. Zarsky, and J. E. Fowler
SEC8, a Subunit of the Putative Arabidopsis Exocyst Complex, Facilitates Pollen Germination and Competitive Pollen Tube Growth
Plant Physiology, August 1, 2005; 138(4): 2005 - 2018.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
D. Monteiro, Q. Liu, S. Lisboa, G. E. F. Scherer, H. Quader, and R. Malho
Phosphoinositides and phosphatidic acid regulate pollen tube growth and reorientation through modulation of [Ca2+]c and membrane secretion
J. Exp. Bot., June 1, 2005; 56(416): 1665 - 1674.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
Z.-l. Shang, L.-g. Ma, H.-l. Zhang, R.-r. He, X.-c. Wang, S.-j. Cui, and D.-y. Sun
Ca2+ Influx into Lily Pollen Grains Through a Hyperpolarization-activated Ca2+-permeable Channel Which Can be Regulated by Extracellular CaM
Plant Cell Physiol., April 1, 2005; 46(4): 598 - 608.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. Takemoto and A. R. Hardham
The Cytoskeleton as a Regulator and Target of Biotic Interactions in Plants
Plant Physiology, December 1, 2004; 136(4): 3864 - 3876.
[Full Text] [PDF]

Home page
 Mol Biol EvolHome page
T. Nagata, S. Iizumi, K. Satoh, H. Ooka, J. Kawai, P. Carninci, Y. Hayashizaki, Y. Otomo, K. Murakami, K. Matsubara, et al.
Comparative Analysis of Plant and Animal Calcium Signal Transduction Element Using Plant Full-Length cDNA Data
Mol. Biol. Evol., October 1, 2004; 21(10): 1855 - 1870.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. Samaj, F. Baluska, B. Voigt, M. Schlicht, D. Volkmann, and D. Menzel
Endocytosis, Actin Cytoskeleton, and Signaling
Plant Physiology, July 1, 2004; 135(3): 1150 - 1161.
[Full Text] [PDF]

Home page
 Plant Physiol.Home page
E. Lobstein, A. Guyon, M. Ferault, D. Twell, G. Pelletier, and S. Bonhomme
The Putative Arabidopsis Homolog of Yeast Vps52p Is Required for Pollen Tube Elongation, Localizes to Golgi, and Might Be Involved in Vesicle Trafficking
Plant Physiology, July 1, 2004; 135(3): 1480 - 1490.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
A. Mazel, Y. Leshem, B. S. Tiwari, and A. Levine
Induction of Salt and Osmotic Stress Tolerance by Overexpression of an Intracellular Vesicle Trafficking Protein AtRab7 (AtRabG3e)
Plant Physiology, January 1, 2004; 134(1): 118 - 128.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
P. J. WHITE and M. R. BROADLEY
Calcium in Plants
Ann. Bot., October 1, 2003; 92(4): 487 - 511.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.