Skip Navigation

This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow E-letters: Submit a response
Right arrow Alert me when this article is cited
Right arrow Alert me when E-letters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in ISI Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (21)
Right arrowRequest Permissions
Right arrow Disclaimer
Google Scholar
Right arrow Articles by Garwe, D.
Right arrow Articles by Mundree, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Garwe, D.
Right arrow Articles by Mundree, S. G.
Agricola
Right arrow Articles by Garwe, D.
Right arrow Articles by Mundree, S. G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Journal of Experimental Botany, Vol. 54, No. 381, pp. 191-201, January 2, 2003
© 2003 Oxford University Press

Molecular characterization of XVSAP1, a stress-responsive gene from the resurrection plant Xerophyta viscosa Baker1

Received 27 May 2002; Accepted 14 August 2002

Dahlia Garwe, Jennifer A. Thomson and Sagadevan G. Mundree2,

Molecular and Cell Biology Department, University of Cape Town, Private Bag, Rondebosch, 7701, South Africa

1 The GenBank Nucleotide Sequence Database Accession Number for the reported nucleotide sequence is AY100455.
2 To whom correspondence should be addressed. Fax: +27 21 689 7573. E-mail: mundree{at}science.uct.ac.za

The strategy of ‘complementation by functional sufficiency’ was used to isolate a cDNA designated XVSAP1 from a cDNA library constructed from dehydrated Xerophyta viscosa Baker leaves. Analysis of the cDNA sequence indicated a highly hydrophobic protein with six transmembrane regions. Southern blot analysis revealed that there are at least two copies of XVSAP1 in X. viscosa. The deduced amino acid sequence showed 49% identity to WCOR413, a low-temperature-regulated protein from wheat. The protein also showed between 25% to 56% identity to WCOR413-like proteins from Arabidopsis thaliana. Expression of XVSAP1 in Escherichia coli (srl::Tn10) conferred osmotic stress tolerance when the cells were grown in 1 M sorbitol. Analysis of gene expression using semi-quantitative RT-PCR indicated that XVSAP1 is induced by dehydration, salt stress (100 mM), both low (4 °C) and high temperature (42 °C) and high light treatment (1500 µmol m–2 s–1). These results suggest that XVSAP1 may have a significant role to play in the response of X. viscosa to abiotic stresses.

Key words: Cold stress, desiccation stress, heat stress, resurrection plant, salinity stress.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J Exp BotHome page
S. Peters, S. G. Mundree, J. A. Thomson, J. M. Farrant, and F. Keller
Protection mechanisms in the resurrection plant Xerophyta viscosa (Baker): both sucrose and raffinose family oligosaccharides (RFOs) accumulate in leaves in response to water deficit
J. Exp. Bot., June 1, 2007; 58(8): 1947 - 1956.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. E. Bartley and B. K. Ishida
Ethylene-sensitive and insensitive regulation of transcription factor expression during in vitro tomato sepal ripening
J. Exp. Bot., June 1, 2007; 58(8): 2043 - 2051.
[Abstract] [Full Text] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.