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Journal of Experimental Botany, Vol. 54, No. 381, pp. 239-248, January 2, 2003
© 2003 Oxford University Press

Sn, a maize bHLH gene, modulates anthocyanin and condensed tannin pathways in Lotus corniculatus

Received 26 June 2002; Accepted 19 August 2002

Mark Paske Robbins3,1, Francesco Paolocci2, John-Wayne Hughes1, Valentina Turchetti2, Gordon Allison1, Sergio Arcioni2, Phillip Morris1 and Francesco Damiani2

1 Cell Biology Department, Institute of Grassland and Environmental Research, Plas Gogerddan, Aberystwyth, Ceredigion SY23 3EB, UK
2 Istituto di Ricerche sul Miglioramento, Genetico della Piante Foraggere, Consiglio Nazionale della Ricerche, Perugia, Italy

3 To whom correspondence should be addressed. Fax: +44 (0)1970 823242. E-mail: mark.robbins{at}bbsrc.ac.uk

Anthocyanins and condensed tannins are major flavonoid end-products in higher plants. While the transactivation of anthocyanins by basic helix-loop-helix (bHLH) transcription factors is well documented, very little is known about the transregulation of the pathway to condensed tannins. The present study analyses the effect of over-expressing an Sn transgene in Lotus corniculatus, a model legume, with the aim of studying the regulation of anthocyanin and tannin end-products. Contrary to expectation, effects on anthocyanin accumulation were subtle and restricted to the leaf midrib, leaf base and petiole tissues. However, the accumulation of condensed tannin polymers was dramatically enhanced in the leaf blade and this increase was accompanied by a 50-fold increase in the number of tannin-containing cells in this tissue. A detailed analysis of selected lines indicated that this transactivational phenotype correlated with high steady-state transcript levels of the introduced transgene and the introduction of a single copy of the CaMV35S-Sn construct into these clonal genotypes. While the levels of condensed tannins in leaves were increased by up to 1% of the dry weight, other major secondary end-products (flavonols, lignins and inducible phytoalexins) were unaltered in transactivated lines. These results give an initial insight into the developmental and higher-order regulation of polyphenolic metabolism in Lotus and other higher plant species.

Key words: Anthocyanins, condensed tannins, Lotus, metabolic engineering, transactivation.


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